Ultrasensitive fluorescent proteins for imaging neuronal activity

被引：4286

作者：

Chen, Tsai-Wen ^{[1
]}

Wardill, Trevor J. ^{[1
]}

Sun, Yi ^{[1
]}

Pulver, Stefan R. ^{[1
]}

Renninger, Sabine L. ^{[2
]}

Baohan, Amy ^{[1
,3
]}

Schreiter, Eric R. ^{[1
]}

Kerr, Rex A. ^{[1
]}

Orger, Michael B. ^{[2
]}

Jayaraman, Vivek ^{[1
]}

Looger, Loren L. ^{[1
]}

Svoboda, Karel ^{[1
]}

Kim, Douglas S. ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Ashburn, VA 20147 USA

[2] Champalimaud Ctr Unknown, Champalimaud Neurosci Programme, P-1400038 Lisbon, Portugal

[3] Univ Calif Los Angeles, Dept Neurobiol, Los Angeles, CA 90095 USA

来源：

NATURE | 2013年 / 499卷 / 7458期

基金：

瑞士国家科学基金会;

关键词：

PRIMARY VISUAL-CORTEX; ENCODED CA2+ INDICATORS; IN-VIVO; NEURAL ACTIVITY; ORIENTATION SELECTIVITY; CORTICAL-NEURONS; CALCIUM TRANSIENTS; TARGET RECOGNITION; DENDRITIC SPINES; STRUCTURAL BASIS;

D O I：

10.1038/nature12354

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo. In layer 2/3 pyramidal neurons of the mouse visual cortex, GCaMP6 reliably detected single action potentials in neuronal somata and orientation-tuned synaptic calcium transients in individual dendritic spines. The orientation tuning of structurally persistent spines was largely stable over timescales of weeks. Orientation tuning averaged across spine populations predicted the tuning of their parent cell. Although the somata of GABAergic neurons showed little orientation tuning, their dendrites included highly tuned dendritic segments (5-40-mm long). GCaMP6 sensors thus provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales.

引用

页码：295 / +

页数：8

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