Fabrication and testing of a PDMS multi-stacked hand-operated LOC for use in portable immunosensing systems

被引：19

作者：

Park, Sin Wook ^{[1
]}

Lee, Jun Hwang ^{[2
]}

Yoon, Hyun C. ^{[2
]}

Kim, Byung Woo ^{[3
]}

Sim, Sang Jun ^{[3
]}

Chae, Heeyeop ^{[3
]}

Yang, Sang Sik ^{[1
]}

机构：

[1] Ajou Univ, Div Elect & Comp Engn, Suwon 441749, South Korea

[2] Ajou Univ, Dept Mol Sci & Technol, Suwon 441749, South Korea

[3] Sungkyunkwan Univ, Dept Chem Engn, Suwon, South Korea

来源：

BIOMEDICAL MICRODEVICES | 2008年 / 10卷 / 06期

关键词：

lab-on-a-chip; immunosensing; hand-operating; surface tension; microfluidics;

D O I：

10.1007/s10544-008-9200-9

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper presents the development of a reliable multi-liquid lab-on-a-chip (LOC), with a hand-operated mechanism, for the application in portable immunosensing systems. To control the transport of multiple liquids without any external equipment, we utilize capillary attraction force for filling and surface tension for stopping liquid flow. As a driving force, hydraulic pressure caused by the elastic deformation of a liquid reservoir transfers liquid stopped at passive valves. The proposed LOC successfully demonstrates a reliable sequential liquid transfer within the reaction channel. To highlight its feasibility as a portable diagnostic system, we performed the electrochemical immunoassay measuring antibody concentrations within the fabricated LOC. As a test biorecognition reaction, the anti-dinitrophenyl (DNP) antibody with an enzymatic catalysis was selected as the target analyte. The amplified signals obtained from this experiment indicated a high selectivity of the immunosensing LOC.

引用

页码：859 / 868

页数：10

共 26 条

[1] Disposable Smart lab on a chip for point-of-care clinical diagnostics [J].

Ahn, CH ;

Choi, JW ;

Beaucage, G ;

Nevin, JH ;

Lee, JB ;

Puntambekar, A ;

Lee, JY .

PROCEEDINGS OF THE IEEE, 2004, 92 (01) :154-173

[2] Immunoassay of infectious agents [J].

Andreotti, PE ;

Ludwig, GV ;

Peruski, AH ;

Tuite, JJ ;

Morse, SS ;

Peruski, LF .

BIOTECHNIQUES, 2003, 35 (04) :850-859

[3] Micro total analysis systems. 2. Analytical standard operations and applications [J].

Auroux, PA ;

Iossifidis, D ;

Reyes, DR ;

Manz, A .

ANALYTICAL CHEMISTRY, 2002, 74 (12) :2637-2652

[4] Microfluidic immunosensor systems [J].

Bange, A ;

Halsall, HB ;

Heineman, WR .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (12) :2488-2503

[5]

BOONE TD, 1998, SOL STAT SENS ACT WO

[6]

CUVAND T, 2004, PHYS FLUIDS, V16, P4575

[7] Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy [J].

Darain, F ;

Park, DS ;

Park, JS ;

Shim, YB .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (10) :1245-1252

[8] Screen-printed microfluidic device for electrochemical immunoassay [J].

Dong, Hua ;

Li, Chang-Ming ;

Zhang, Yi-Fan ;

Cao, Xiao-Dong ;

Gan, Ye .

LAB ON A CHIP, 2007, 7 (12) :1752-1758

[9] Study on highly sensitive potentiometric IgG immunosensor [J].

Feng, CL ;

Xu, YH ;

Song, LM .

SENSORS AND ACTUATORS B-CHEMICAL, 2000, 66 (1-3) :190-192

[10] The pressure drop along rectangular microchannels containing bubbles [J].

Fuerstman, Michael J. ;

Lai, Ann ;

Thurlow, Meghan E. ;

Shevkoplyas, Sergey S. ;

Stone, Howard A. ;

Whitesides, George M. .

LAB ON A CHIP, 2007, 7 (11) :1479-1489

← 1 2 3 →