Electrochemical immunoassay of human chorionic gonadotrophin based on its immobilization in gold nanoparticles-chitosan membrane

被引：28

作者：

Chen, J

Yan, F

Du, D

Wu, J

Ju, HX ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, Educ Minist China, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

[2] Jiangsu Inst Canc Prevent & Cure, Nanjing 210009, Peoples R China

来源：

ELECTROANALYSIS | 2006年 / 18卷 / 07期

关键词：

immunosensors; immunoassay; gold nanoparticles; chitosan; human chorionic gonadotrophin;

D O I：

10.1002/elan.200503440

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A human chorionic gonadotrophin (hCG) doped gold nanoparticles-chitosan membrane was prepared for forming an immunoconjugate of horseradish peroxidase labeled hCG antibody and hCG on glassy carbon electrode. The nanoparticles provided a congenial environment of the adsorbed proteins. Thus, the immobilized HRP-labeled immunoconjugate showed good enzymatic activity for the oxidation of o-phenylenediamine by H2O2. With a competitive mechanism, an amperometric method for immunoassay of hCG up to 30 mIU mL(-1) with a relatively low detection limit of 0.26 mIU mL(-1) at 3 sigma was developed. The hCG immunosensor showed good precision, high sensitivity, acceptable stability and reproducibility.

引用

页码：670 / 676

页数：7

共 38 条

[21] New electrochemical assay of alkaline phosphatase using ascorbic acid 2-phosphate and its application to enzyme immunoassay [J].