Colony multiplex PCR assay for identification and differentiation of Campylobacter jejuni, C-coli, C-lari, C-upsaliensis, and C-fetus subsp fetus

被引：404

作者：

Wang, GH ^{[1
]}

Clark, CG ^{[1
]}

Taylor, TM ^{[1
]}

Pucknell, C ^{[1
]}

Barton, C ^{[1
]}

Price, L ^{[1
]}

Woodward, DL ^{[1
]}

Rodgers, FG ^{[1
]}

机构：

[1] Natl Microbiol Lab, Natl Lab Enter Pathogens, Special Project Unit, Winnipeg, MB R3E 3R2, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 12期

关键词：

D O I：

10.1128/JCM.40.12.4744-4747.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiplex PCR assay was used to simultaneously detect genes from the five major clinically relevant Campylobacter spp. Those genes selected were hipO and 23S rRNA from Campylobacter jejuni; glyA from each of C. coli, C. lari, and C. upsaliensis; and sapB2 from C.fetus subsp.fetus. The assay was evaluated with 137 clinical and environmental isolates and was found to be rapid and easy to perform and had a high sensitivity and specificity for characterizing isolates, even in mixed cultures.

引用

页码：4744 / 4747

页数：4

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