Determination of glutathione in spruce needles by liquid chromatography/tandem mass spectrometry

被引：25

作者：

Gucek, M

Makuc, S

Mlakar, A

Bericnik-Vrbovsek, J

Marsel, J

机构：

[1] Univ Ljubljana, Fac Chem & Chem Technol, SI-1000 Ljubljana, Slovenia

[2] KRKA dd, SI-8501 Novo Mesto, Slovenia

[3] ERICo Velenje, Inst Ecol Res, SI-3220 Velenje, Slovenia

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2002年 / 16卷 / 12期

关键词：

D O I：

10.1002/rcm.700

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For the determination of glutathione (GSH) and its oxidized form (GSSG) in spruce needles their electrospray mass and MS/MS spectra were recorded with an ion trap mass spectrometer (ITMS, LCQ Finnigan) and a triple stage quadrupole mass spectrometer (TSQ Quattro II, Micromass). A study of the stability of GSH in aqueous solutions shows the presence of dimeric and trimeric forms of GSH, as well as GSSG, GSH-sulfonate and GSH-sulfinic acid. The same components were also found in extracts of spruce needles. We developed an assay which is suitable for monitoring low concentrations of GSH and similar compounds in plant tissues, employing the sensitivity and specificity of LC/MS/MS. Preliminary results on the mass spectrometric determination of GSH in spruce needles are given. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：1186 / 1191

页数：6

共 13 条

[1] Simultaneous determination of reduced and oxidized glutathione in peripheral blood mononuclear cells by liquid chromatography-electro spray mass spectrometry [J].