A novel angiotensin I-converting enzyme inhibitory peptide from Phascolosoma esculenta water-soluble protein hydrolysate

被引：37

作者：

Du, Lei ^{[2
]}

Fang, Ming ^{[2
]}

Wu, Hongxi ^{[3
]}

Xie, Jingli ^{[1
,2
]}

Wu, Yanling ^{[2
]}

Li, Peng ^{[2
]}

Zhang, Daihui ^{[2
]}

Huang, Zhenhua ^{[3
]}

Xia, Yuelan ^{[1
]}

Zhou, Li ^{[1
]}

Wei, Dongzhi ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] E China Univ Sci & Technol, Dept Food Sci & Engn, Shanghai 200237, Peoples R China

[3] Zhejiang Mariculture Res Inst, Zhejiang Key Lab Coastal Bioresource, Wenzhou 325005, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2013年 / 5卷 / 01期

关键词：

Phascolosoma esculenta; Angiotensin I-converting enzyme inhibitor; Peptide; Purification; Spontaneously hypertensive rats; FRAME PROTEIN; PURIFICATION; IDENTIFICATION; HYPERTENSION;

D O I：

10.1016/j.jff.2012.12.003

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

The water-soluble protein from Phascolosoma esculenta was hydrolyzed by pepsin to obtain the hydrolysate with angiotensin I-converting enzyme (ACE) inhibitory activity. The hydrolysate (PEPH) was then further separated by membrane bioreactor system, ion-exchange chromatography, gel filtration, and reversed phase high-performance liquid chromatography (RP-HPLC) and a novel ACE inhibitory peptide named as PeP with the IC50 value of 135 M was isolated. The amino acid sequence, Ala-Trp-Leu-His-Pro-Gly-Ala-Pro-Lys-Val-Phe, was identified by matrix-assisted laser desorption ionization-time of flight/time of flight (MALDI-TOF/TOF). Inhibitory kinetics study suggested that PeP acted as competitive inhibitor against ACE. Single oral administration of synthesized PeP at 10 mg/kg dose in spontaneously hypertensive rats could reduce the systolic blood pressure around 30 mmHg and the effect could last for more than 8 h. The results suggest that peptide from P. esculenta could be a potent natural ingredient for functional foods or pharmaceuticals against hypertension. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：475 / 483

页数：9

共 36 条

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Structural analysis of new antihypertensive peptides derived from cheese whey protein by proteinase K digestion [J].