Surface plasmon resonance detection of E. coli and methicillin-resistant S. aureus using bacteriophages

被引:160
作者
Tawil, Nancy [1 ,2 ,3 ]
Sacher, Edward [1 ]
Mandeville, Rosemonde [2 ]
Meunier, Michel [3 ]
机构
[1] Ecole Polytech, Dept Genie Phys, Regrp Quebecois Mat Pointe, Montreal, PQ H3C 3A7, Canada
[2] Biophage Pharma Inc, Montreal, PQ H4P 2R2, Canada
[3] Ecole Polytech, Dept Genie Phys, Laser Proc & Plasmon Lab, Montreal, PQ H3C 3A7, Canada
关键词
Bacteriophage; Biosensor; Methicillin-resistant Staphylococcus aureus (MRSA); Surface plasmon resonance (SPR); STAPHYLOCOCCUS-AUREUS; PHAGE; ADSORPTION; BIOSENSORS; PROBE;
D O I
10.1016/j.bios.2012.04.048
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Early diagnosis and appropriate treatment of Escherichia coli (E. coli) O157:H7 and methicillin-resistant Staphylococcus aureus (MRSA) are key elements in preventing resultant life-threatening illnesses, such as hemorrhagic colitis, hemolytic uremic syndrome, and septicemia. In this report, we describe the use of surface plasmon resonance (SPR) for the biodetection of pathogenic bacteria, using bacteriophages as the recognition elements. T4 bacteriophages were used to detect E. coli, while a novel, highly specific phage was used to detect MRSA. We found that the system permits label-free, real-time, specific, rapid and cost-effective detection of pathogens, for concentrations of 10(3) colony forming units/milliliter, in less than 20min. This system promises to become a diagnostic tool for bacteria that cause major public concern for food safety, bioterrorism, and nosocomial infections. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:24 / 29
页数:6
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