Higher-throughput approaches to crystallization and crystal structure determination

被引:53
作者
Fogg, Mark J. [1 ]
Wilkinson, Anthony J. [1 ]
机构
[1] Univ York, Struct Biol Lab, Dept Chem, York YO10 5YW, N Yorkshire, England
关键词
crystallization; high-throughput structural determination; ligation-independent cloning; protein crystallography; structural genomics;
D O I
10.1042/BST0360771
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In recent times, there has been a large increase in the number of protein structures deposited in the Protein Data Bank. Structural genomics initiatives have contributed to this expansion through their focus on high-throughput structural determination. This has fuelled advances in many of the techniques in the pipeline from gene to protein to crystal to structure. These include ligation-independent cloning methods, parallel purification systems, robotic crystallization devices and automated methods of crystal identification, data collection and, in some cases, structure solution. Some of these advances are described and discussed briefly with an emphasis on activities in the York Structural Biology Laboratory through its participation in the Structural Proteomics in Europe consortium.
引用
收藏
页码:771 / 775
页数:5
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