Automation of sample mounting for macromolecular crystallography

被引：109

作者：

Cipriani, F.

Felisaz, F.

Launer, L.

Aksoy, J. -S.

Caserotto, H.

Cusack, S.

Dallery, M.

di-Chiaro, F.

Guijarro, M.

Huet, J.

Larsen, S.

Lentini, Mario

McCarthy, J.

McSweeney, S.

Ravelli, R.

Renier, M.

Taffut, C.

Thompson, A.

Leonard, G. A.

Walsh, M. A.

机构：

[1] EMBL Grenoble, F-38042 Grenoble 9, France

[2] MRC, ESRF, F-38043 Grenoble, France

[3] European Synchrotron Radiat Facil, F-38043 Grenoble, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2006年 / 62卷

关键词：

D O I：

10.1107/S0907444906030587

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A standard sample holder and vial for cryocooled macromolecular crystals has been defined for use with robotic sample changers. This SPINE standard sample holder is a modified version, with added features and specifications, of sample holders in common use. In particular, the SPINE standard meets the precision required for automatic sample exchange and includes a cap that is identified by a two-dimensional datamatrix code as well as an optional vial. At the ESRF, the sample holder standard is in use with the EMBL/ ESRF/ BM14 robotic sample changer ( SC3) which is installed on eight beamlines. The SC3 can hold up to 50 crystals stored in five baskets. A datamatrix reader in the SC3 ensures safe management of the sample flow and facilitates fully automatic screening and characterization of samples. Tools for handling and transporting 50 samples in a dry shipping dewar have been developed. In addition to the SC3, the SPINE sample holder is currently compatible with a number of other robotic sample changers.

引用

页码：1251 / 1259

页数：9

共 12 条

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