SPINE high-throughput crystallization, crystal imaging and recognition techniques: current state, performance analysis, new technologies and future aspects

被引:47
作者
Berry, Ian M.
Dym, O.
Esnouf, R. M.
Harlos, K.
Meged, R.
Perrakis, A.
Sussman, J. L.
Walter, T. S.
Wilson, J.
Messerschmidt, Albrecht
机构
[1] Max Planck Inst Biochem, Dept Proteom & Signal Transduct, D-82152 Martinsried, Germany
[2] Wellcome Trust Ctr Human Genet, Div Struct Biol, Oxford, England
[3] Wellcome Trust Ctr Human Genet, Oxford Prot Prod Facil, Oxford, England
[4] Weizmann Inst Sci, Dept Struct Chem, Israel Struct Proteom Ctr, IL-76100 Rehovot, Israel
[5] Netherlands Canc Inst, Div Mol Carcinogenesis, NL-1066 CX Amsterdam, Netherlands
[6] Univ York, Dept Chem, York Struct Biol Lab, York YO10 5YW, N Yorkshire, England
来源
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2006年 / 62卷
基金
英国医学研究理事会;
关键词
D O I
10.1107/S090744490602943X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper reviews the developments in high- throughput and nanolitre- scale protein crystallography technologies within the remit of workpackage 4 of the Structural Proteomics In Europe ( SPINE) project since the project's inception in October 2002. By surveying the uptake, use and experience of new technologies by SPINE partners across Europe, a picture emerges of highly successful adoption of novel working methods revolutionizing this area of structural biology. Finally, a forward view is taken of how crystallization methodologies may develop in the future.
引用
收藏
页码:1137 / 1149
页数:13
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