Functional protein microarrays

被引：149

作者：

Wilson, DS ^{[1
]}

Nock, S ^{[1
]}

机构：

[1] Zyomyx Inc, Hayward, CA 94545 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2002年 / 6卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(01)00281-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microarrays of immobilized functional proteins have the potential to increase dramatically the throughput of proteomic analysis. Micro-immunoassays, in which biological samples are exposed to arrays of immobilized antibodies, can be used for protein expression profiling. In addition, protein function can be elucidated by performing binding and enzymatic assays on arrays of biologically active proteins.

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页码：81 / 85

页数：5

相关论文

共 44 条

[1] Protein microchips: Use for immunoassay and enzymatic reactions [J].

Arenkov, P ;

Kukhtin, A ;

Gemmell, A ;

Voloshchuk, S ;

Chupeeva, V ;

Mirzabekov, A .

ANALYTICAL BIOCHEMISTRY, 2000, 278 (02) :123-131

[2]

Belov L, 2001, CANCER RES, V61, P4483

[3] Micromosaic immunoassays [J].

Bernard, A ;

Michel, B ;

Delamarche, E .

ANALYTICAL CHEMISTRY, 2001, 73 (01) :8-12

[4] Protein patterning [J].

Blawas, AS ;

Reichert, WM .

BIOMATERIALS, 1998, 19 (7-9) :595-609

[5] The use of aptamers in large arrays for molecular diagnostics [J].

Brody, EN ;

Willis, MC ;

Smith, JD ;

Jayasena, S ;

Zichi, D ;

Gold, L .

MOLECULAR DIAGNOSIS, 1999, 4 (04) :381-388

[6]

Butler J. E., 1993, Molecular Immunology, V30, P1165, DOI 10.1016/0161-5890(93)90135-X

[7] THE PHYSICAL AND FUNCTIONAL-BEHAVIOR OF CAPTURE ANTIBODIES ADSORBED ON POLYSTYRENE [J].

BUTLER, JE ;

NI, L ;

NESSLER, R ;

JOSHI, KS ;

SUTER, M ;

ROSENBERG, B ;

CHANG, J ;

BROWN, WR ;

CANTARERO, LA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 150 (1-2) :77-90

[8] Protein and antibody arrays and their medical applications [J].

Cahill, DJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 2001, 250 (1-2) :81-91

[9] Exploring the metabolic and genetic control of gene expression on a genomic scale [J].

DeRisi, JL ;

Iyer, VR ;

Brown, PO .

SCIENCE, 1997, 278 (5338) :680-686

[10] Site-specific attachment of functionalized poly(ethylene glycol) to the amino terminus of proteins [J].

Gaertner, HF ;

Offord, RE .

BIOCONJUGATE CHEMISTRY, 1996, 7 (01) :38-44

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