Surface Functionalization of Living Cells with Multilayer Patches

被引：166

作者：

Swiston, Albert J. ^{[1
]}

Cheng, Connie ^{[4
]}

Um, Soong Ho ^{[1
,2
]}

Irvine, Darrell J. ^{[1
,2
]}

Cohen, Robert E. ^{[3
]}

Rubner, Michael F. ^{[1
]}

机构：

[1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA

[2] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[4] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

来源：

NANO LETTERS | 2008年 / 8卷 / 12期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1021/nl802404h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate that functional polyelectrolyte multilayer (PEM) patches can be attached to a fraction of the surface area of living, individual lymphocytes. Surf ace-modified cells remain viable at least 48 h following attachment of the functional patch, and patches carrying magnetic nanoparticles allow the cells to be spatially manipulated using a magnetic field. The patch does not completely occlude the cellular surface from the surrounding environment; this approach allows a functional payload to be attached to a cell that is still free to perform its native functions, as suggested by preliminary studies on patch-modified T-cell migration. This approach has potential for broad applications in bioimaging, cellular functionalization, immune system and tissue engineering, and cell-based therapeutics where cell-environment interactions are critical.

引用

收藏

页码：4446 / 4453

页数：8

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