Integron- and carbenicillinase-mediated reduced susceptibility to amoxicillin-clavulanic acid in isolates of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 from French patients

被引:59
作者
Poirel, L
Guibert, M
Bellais, S
Naas, T
Nordmann, P
机构
[1] Hop Bicetre, Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France
[2] Hop Antoine Beclere, Serv Bacteriol Virol, F-92141 Clamart, France
[3] Assistance Publ Hop Paris, Fac Med Paris Sud, Paris, France
关键词
D O I
10.1128/AAC.43.5.1098
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Fifty-seven Salmonella enterica serotype Typhimurium (S. typhimurium) isolates were collected from human patients in two French hospitals, Hopital Antoine Beclere (Clamart, France) and Hopital Bicetre (Le Kremlin-Bicetre, France), between 1996 and 1997. Thirty of them (52 percent) were resistant to amino-, carbeni-, and ureidopenicillins, had reduced susceptibility to amoxicillin-clavulanic acid, were susceptible to cephalothin, and were resistant to sulfonamides, streptomycin, chloramphenicol, and tetracyclines. All these strains possessed a bla(PSE-1)-like gene and were of phage type DT104. Ten of them were studied in more detail, which revealed that bla(PSE-1) is located on the variable region of a class 1 integron. This integron was found to be chromosomally located, as was another class 1 integron containing aadA2, a streptomycin-spectinomycin resistance gene. The reduced susceptibility to amoxicillin-clavulanic acid (and to ticarcillin-clavulanic acid) may result from the high level of hydrolysis of the p-lactam rather than to the clavulanic acid resistance properties of PSE-1 in these clonally related S. typhimurium isolates.
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页码:1098 / 1104
页数:7
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