The soluble sperm factor that causes Ca2+ release from sea-urchin (Lytechinus pictus) egg homogenates also triggers Ca2+ oscillations after injection into mouse eggs

被引：50

作者：

Parrington, J

Jones, KT

Lai, FA

Swann, K

机构：

[1] UCL, Dept Anat, London WC1E 6BT, England

[2] Univ Coll N Wales, Coll Med, Cardiff CF4 4XN, S Glam, Wales

来源：

BIOCHEMICAL JOURNAL | 1999年 / 341卷

基金：

英国惠康基金;

关键词：

calcium; phospholipase C;

D O I：

10.1042/0264-6021:3410001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytosolic extracts of boar sperm contain a soluble phospholipase C (PLC) activity that induces Ca2+ release in sea-urchin (Lytechinus pictus) egg homogenates and an uncharacterized protein factor that causes Ca2+ oscillations when injected into mammalian eggs. In the present study we fractionated boar sperm extracts on three different FPLC chromatographic columns and found that the fractions that caused maximal Ca2+ release in sea-urchin egg homogenates were also the ones that triggered Ca2+ oscillations in mouse eggs. Our data suggests that the sperm factor which triggers Ca2+ oscillations in eggs contains a PLC and not the 33 kDa glucosamine deaminase preciously suggested to be one its components.

引用

页码：1 / 4

页数：4

共 40 条

[1]

Caramelli E, 1996, EUR J CELL BIOL, V71, P154

[2]

CIAPA B, 1992, DEVELOPMENT, V115, P187

[3]

CLAPPER DL, 1985, J BIOL CHEM, V260, P3947

[4] Phospholipase C in mouse oocytes: Characterization of beta and gamma isoforms and their possible involvement in sperm-induced Ca2+ spiking [J].