A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca2+ release in sea urchin egg homogenates

Injection of sperm extracts triggers Ca2+ oscillations in mammalian eggs similar to those seen at fertilisation, Here, we show that addition of sperm extracts to sea urchin egg homogenates causes Ca2+ release and inositol 1,4,5-trisphosphate (InsP(3)) production. Furthermore depleting homogenates of phosphatidylinositol lipids using a phosphatidylinositol-specific phospholipase C blocked the sperm extract from causing InsP(3) production and a Ca2+ rise. A response could be recovered by the addition of phosphatidylinositol 4,5-bisphosphate to either sperm extracts or egg homogenates. These data indicate that sperm extracts contain an InsP(3)-generating phospholipase C which may play a role in Ca2+ release at fertilisation, (C) 1998 Federation of European Biochemical Societies.