Microspore embryogenesis and the development of a double haploidy protocol for cow cockle (Saponaria vaccaria)

被引：23

作者：

Kernan, Z ^{[1
]}

Ferrie, AMR ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

来源：

PLANT CELL REPORTS | 2006年 / 25卷 / 04期

关键词：

cow cockle; haploids; microspore culture; microspore embryogenesis; Saponaria vaccaria;

D O I：

10.1007/s00299-005-0064-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Factors affecting microspore embryogenesis of cow cockle (Saponaria vaccaria) were evaluated including donor plant growing conditions, genotype, bud size, density, medium composition, and culture conditions. Of the two donor plant (day/night) temperature regimes evaluated (10/5 degrees C and 20/15 degrees C), plants grown at 20/15 degrees C were the most embryogenic. An embryogenic frequency of greater than 350 embryos/100 buds was observed in the most embryogenic genotype, cv. 'White Beauty'. Buds from 3-9 mm in length were evaluated for their embryogenic potential; buds that were 4-7.9 mm produced the most embryos/100 buds. Of all the media compositions evaluated, NLN medium with 15% sucrose resulted in the most embryos. Cow cockle microspores required an initial period of 32 degrees C for 3 days for production of microspore-derived embryos (MDEs).

引用

页码：274 / 280

页数：7

共 44 条

[21] What can nature offer from an emulsifier point of view: trends and progress? [J].

Garti, N .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 1999, 152 (1-2) :125-146

[22] UTILIZATION AND AGRONOMIC STUDIES OF COW COCKLE (SAPONARIA VACCARIA) [J].

GOERING, KJ ;

ESLICK, RF ;

WATSON, CA ;

KENG, J .

ECONOMIC BOTANY, 1966, 20 (04) :429-&

[23]

HAMAOKA Y, 1991, PHYSIOL PLANTARUM, V82, P67, DOI 10.1111/j.1399-3054.1991.tb02903.x

[24] EFFECT OF GYPSOGENIN 3,O-GLUCURONIDE PRETREATMENT OF GYPSOPHILA-PANICULATA AND SAPONARIA-OFFICINALIS CELL-SUSPENSION CULTURES ON THE ACTIVITIES OF MICROSOMAL 2,3-OXIDOSQUALENE CYCLOARTENOL AND AMYRIN CYCLASES [J].

HENRY, M ;

RAHIER, A ;

TATON, M .

PHYTOCHEMISTRY, 1992, 31 (11) :3855-3859

[25]

Hostettmann K., 1995, SAPONINS

[26] EFFECTS OF CULTURE DENSITY, CONDITIONED MEDIUM AND FEEDER CULTURES ON MICROSPORE EMBRYOGENESIS IN BRASSICA-NAPUS L CV TOPAS [J].

HUANG, B ;

BIRD, S ;

KEMBLE, R ;

SIMMONDS, D ;

KELLER, W ;

MIKI, B .

PLANT CELL REPORTS, 1990, 8 (10) :594-597

[27]

Hunter CP, 1988, THESIS U LONDON LOND

[28]

Kasha K.J., 2003, Doubled haploid production in crop plants, P77, DOI DOI 10.1007/978-94-017-1293-4_13

[29] An improved in vitro technique for isolated microspore culture of barley [J].

Kasha, KJ ;

Simion, E ;

Oro, R ;

Yao, QA ;

Hu, TC ;

Carlson, AR .

EUPHYTICA, 2001, 120 (03) :379-385

[30]

Keller W., 1987, P 7 INT RAP C

← 1 2 3 4 5 →