Heritable genome editing in C. elegans via a CRISPR-Cas9 system

被引：657

作者：

Friedland, Ari E. ^{[1
]}

Tzur, Yonatan B. ^{[1
]}

Esvelt, Kevin M. ^{[2
]}

Colaiacovo, Monica P. ^{[1
]}

Church, George M. ^{[1
,2
]}

Calarco, John A. ^{[3
]}

机构：

[1] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Cambridge, MA 02138 USA

[3] Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USA

来源：

NATURE METHODS | 2013年 / 10卷 / 08期

基金：

美国国家卫生研究院;

关键词：

CAENORHABDITIS-ELEGANS; GENE;

D O I：

10.1038/nmeth.2532

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

引用

页码：741 / +

页数：5

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