miR-145 suppresses osteogenic differentiation by targeting Sp7

被引：121

作者：

Jia, Jie ^{[1
]}

Tian, Qing ^{[1
]}

Ling, Song ^{[1
]}

Liu, Yong ^{[1
]}

Yang, Shuhua ^{[1
]}

Shao, Zengwu ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Orthoped, Union Hosp, Tongji Med Coll, Wuhan 430022, Hubei, Peoples R China

来源：

FEBS LETTERS | 2013年 / 587卷 / 18期

关键词：

miR-145; Osteogenesis; Differentiation; Sp7; TRANSCRIPTION FACTOR OSTERIX; OSTEOBLAST DIFFERENTIATION; CANCER CELLS; EXPRESSION; PROMOTES; PROLIFERATION;

D O I：

10.1016/j.febslet.2013.07.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic differentiation. miR-145 was decreased during osteogenic differentiation, which could suppress the osteogenic differentiation of C2C12 and MC3T3-E1 cells confirmed by gain- and loss-of-function experiments. Moreover, bioinformatic analysis combined with luciferase reporter assay, and Western blot validated that miR-145 negatively regulated Sp7 expression. Inhibition of Sp7 showed similar effect with miR-145 on osteogenic differentiation, whereas overexpression of Sp7 attenuated this effect. Collectively, these data indicate that miR-145 is a novel regulator of Sp7, and it suppresses the osteogenic differentiation of C2C12 and MC3T3-E1 cells. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3027 / 3031

页数：5

共 27 条

[1]

MicroRNA-322 (miR-322) and Its Target Protein Tob2 Modulate Osterix (Osx) mRNA Stability [J].