Neuron-specific expression of neuroglobin in mammals

被引:68
作者
Laufs, TL
Wystub, S
Reuss, S
Burmester, T
Saaler-Reinhardt, S
Hankeln, T
机构
[1] Johannes Gutenberg Univ Mainz, Inst Mol Genet Biosafety Res & Consulting, D-6500 Mainz, Germany
[2] Johannes Gutenberg Univ Mainz, Sch Med, Dept Anat, D-6500 Mainz, Germany
[3] Johannes Gutenberg Univ Mainz, Inst Zool, D-6500 Mainz, Germany
[4] Johannes Gutenberg Univ Mainz, Inst Physiol Chem, D-6500 Mainz, Germany
关键词
globin; neuroglobin; oxygen-binding; glial cells; gene expression;
D O I
10.1016/j.neulet.2004.02.072
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neuroglobin, a vertebrate oxygen-binding protein, is expressed in many regions of the adult brain. We examined the cell type-specific expression of neuroglobin in neurons and astroglial cells in primary cultures of fetal hippocampal cells and sections of the adult mouse brain using neuroglobin-specific polyclonal antibodies and cell type-specific markers NeuN and GFAP to differentiate between neurons and glial cells. Neuroglobin is exclusively expressed in neurons, but not in astroglial cells. Accordingly, neuroglobin was detected in two neuroblastoma cell lines (N2a, SH-SY5Y) and the pheochromocytoma cell line PC-12, but not in glioblastoma cell lines (DKMG, GAMG) or other, non-neural cells (HeLa, Vero). Analysis of the neuroglobin genomic sequence from man and mouse identifies sequence motifs with similarity to the neuron-restrictive silencer element, possibly explaining a neuron-specific expression of neuroglobin. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:83 / 86
页数:4
相关论文
共 25 条
[1]  
ALKONDON M, 1994, J PHARMACOL EXP THER, V271, P494
[2]   A vertebrate globin expressed in the brain [J].
Burmester, T ;
Weich, B ;
Reinhardt, S ;
Hankeln, T .
NATURE, 2000, 407 (6803) :520-523
[3]  
Coulson JM, 1999, CANCER RES, V59, P5123
[4]   Biochemical characterization and ligand binding properties of neuroglobin, a novel member of the globin family [J].
Dewilde, S ;
Kiger, L ;
Burmester, T ;
Hankeln, T ;
Baudin-Creuza, V ;
Aerts, T ;
Marden, MC ;
Caubergs, R ;
Moens, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (42) :38949-38955
[5]   Globin and globin gene structure of the nerve myoglobin of Aphrodite aculeata [J].
Dewilde, S ;
Blaxter, M ;
VanHauwaert, ML ;
Vanfleteren, J ;
Esmans, EL ;
Marden, M ;
Griffon, N ;
Moens, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (33) :19865-19870
[6]   A globin in the nucleus! [J].
Geuens, E ;
Brouns, I ;
Flamez, D ;
Dewilde, S ;
Timmermans, JP ;
Moens, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (33) :30417-30420
[7]   The redox state of the cell regulates the ligand binding affinity of human neuroglobin and cytoglobin [J].
Hamdane, D ;
Kiger, L ;
Dewilde, S ;
Green, BN ;
Pesce, A ;
Uzan, J ;
Burmester, T ;
Hankeln, T ;
Bolognesi, M ;
Moens, L ;
Marden, MC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (51) :51713-51721
[8]   EXTENDED OXYGEN DELIVERY FROM THE NERVE HEMOGLOBIN OF TELLINA-ALERNATA (BIVALVIA) [J].
KRAUS, DW ;
COLACINO, JM .
SCIENCE, 1986, 232 (4746) :90-92
[9]   RE-1 silencing transcription factor (REST) regulates human synaptophysin gene transcription through an intronic sequence-specific DNA-binding site [J].
Lietz, M ;
Hohl, M ;
Thiel, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (01) :2-9
[10]   Neuroglobin, a novel member of the globin family, is expressed in focal regions of the brain [J].
Mammen, PPA ;
Shelton, JM ;
Goetsch, SC ;
Williams, SC ;
Richardson, JA ;
Garry, MG ;
Garry, DJ .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2002, 50 (12) :1591-1598