Generation of new human embryonic stem cell lines with diploid and triploid karyotypes

被引：136

作者：

Baharvand, H ^{[1
]}

Ashtiani, SK

Taee, A

Massumi, M

Valojerdi, MR

Yazdi, PE

Moradi, SZ

Farrokhi, A

机构：

[1] Royan Inst, Dept Stem Cells, Tehran, Iran

[2] Royan Inst, Dept Embryol, Tehran, Iran

[3] Royan Inst, Dept Genet, Tehran, Iran

来源：

DEVELOPMENT GROWTH & DIFFERENTIATION | 2006年 / 48卷 / 02期

关键词：

colony morphology; differentiation; doubling time; embryonic stem cell; human embryo; stemness genes;

D O I：

10.1111/j.1440-169x.2006.00851.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human pluripotent embryonic stem cells (hESC) have great promise for research into human developmental biology and the development of cell therapies for the treatment of diseases. To meet the increased demand for characterized hESC lines, we present the derivation and characterization of five hESC lines on mouse embryonic fibroblast cells. Our stem cell lines are characterized by morphology, long-term expansion, and expression profiles of a number of specific markers, including TRA-1-60, TRA-1-81, alkaline phosphatase, connexin 43, OCT-4, NANOG, CXCR4, NODAL, LEFTY2, THY-1, TDGF1, PAX6, FOXD3, SOX2, EPHA2, FGF4, TAL1, AC133 and REX-1. The pluripotency of the cell line was confirmed by spontaneous differentiation under in vitro conditions. Whereas all of the cell lines expressed all the characteristics of undifferentiated pluripotent hESC, two of the cell lines carried a triploid karyotype.

引用

页码：117 / 128

页数：12

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