Single-molecule investigation of G-quadruplex using a nanopore sensor

被引：29

作者：

Shim, Jiwook ^{[1
,2
]}

Gu, Li-Qun ^{[3
,4
]}

机构：

[1] Univ Illinois, Micro & Nanotechnol Lab, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA

[3] Univ Missouri, Dept Biol Engn, Columbia, MO 65211 USA

[4] Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA

来源：

METHODS | 2012年 / 57卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Nanopore; Alpha-hemolysin; G-quadruplex; Thrombin-binding aptamer; Folding and unfolding; Kinetics; STAPHYLOCOCCAL ALPHA-HEMOLYSIN; PORE-FORMING PROTEIN; DNA APTAMER; TELOMERIC REPEAT; NUCLEIC-ACIDS; BINDING; SEQUENCE; ION; IDENTIFICATION; BASE;

D O I：

10.1016/j.ymeth.2012.03.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review article introduces the nanopore single-molecule method for the study of G-quadruplex nucleic acid structures. Single G-quadruplexes can be trapped into a 2 nm protein pore embedded in the lipid bilayer membrane. The trapped G-quadruplex specifically blocks the current through the nanopore, creating a signature event for quantitative analysis of G-quadruplex properties, from cation-determined folding and unfolding kinetics to the interactions with the protein ligand. The nanopore single-molecule method is simple, accurate, and requires no labels. It can be used to evaluate G-quadruplex mechanisms and it may have applications in G-quadruplex-based biosensors, nanomachines, and nanostructure assembly. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：40 / 46

页数：7

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