Single-molecule investigation of G-quadruplex using a nanopore sensor

被引：29

作者：

Shim, Jiwook ^{[1
,2
]}

Gu, Li-Qun ^{[3
,4
]}

机构：

[1] Univ Illinois, Micro & Nanotechnol Lab, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA

[3] Univ Missouri, Dept Biol Engn, Columbia, MO 65211 USA

[4] Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA

来源：

METHODS | 2012年 / 57卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Nanopore; Alpha-hemolysin; G-quadruplex; Thrombin-binding aptamer; Folding and unfolding; Kinetics; STAPHYLOCOCCAL ALPHA-HEMOLYSIN; PORE-FORMING PROTEIN; DNA APTAMER; TELOMERIC REPEAT; NUCLEIC-ACIDS; BINDING; SEQUENCE; ION; IDENTIFICATION; BASE;

D O I：

10.1016/j.ymeth.2012.03.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review article introduces the nanopore single-molecule method for the study of G-quadruplex nucleic acid structures. Single G-quadruplexes can be trapped into a 2 nm protein pore embedded in the lipid bilayer membrane. The trapped G-quadruplex specifically blocks the current through the nanopore, creating a signature event for quantitative analysis of G-quadruplex properties, from cation-determined folding and unfolding kinetics to the interactions with the protein ligand. The nanopore single-molecule method is simple, accurate, and requires no labels. It can be used to evaluate G-quadruplex mechanisms and it may have applications in G-quadruplex-based biosensors, nanomachines, and nanostructure assembly. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：40 / 46

页数：7

共 100 条

[41] Temperature-Independent Porous Nanocontainers for Single-Molecule Fluorescence Studies [J].

Ishitsuka, Yuji ;

Okumus, Burak ;

Arslan, Sinan ;

Chen, Kok Hao ;

Ha, Taekjip .

ANALYTICAL CHEMISTRY, 2010, 82 (23) :9694-9701

[42] G-Quadruplex DNA Bound by a Synthetic Ligand is Highly Dynamic [J].

Jena, Prakrit V. ;

Shirude, Pravin S. ;

Okumus, Burak ;

Laxmi-Reddy, Katta ;

Godde, Frederic ;

Huc, Ivan ;

Balasubramanian, Shankar ;

Ha, Taekjip .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (35) :12522-+

[43] Structure-activity of tetrad-forming oligonucleotides as a potent anti-HIV therapeutic drug [J].

Jing, NJ ;

Hogan, ME .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (52) :34992-34999

[44] Ion selective folding of loop domains in a potent anti-HIV oligonucleotide [J].

Jing, NJ ;

Rando, RF ;

Pommier, Y ;

Hogan, ME .

BIOCHEMISTRY, 1997, 36 (41) :12498-12505

[45] The internal cavity of the staphylococcal α-hemolysin pore accommodates ∼175 exogenous amino acid residuest [J].

Jung, Y ;

Cheley, S ;

Braha, O ;

Bayley, H .

BIOCHEMISTRY, 2005, 44 (25) :8919-8929

[46] G-quadruplex formation in human telomeric (TTAGGG)4 sequence with complementary strand in close vicinity under molecularly crowded condition [J].

Kan, Zhong-Yuan ;

Lin, Yi ;

Wang, Feng ;

Zhuang, Xin-Ying ;

Zhao, Yong ;

Pang, Dai-Wen ;

Hao, Yu-Hua ;

Tan, Zheng .

NUCLEIC ACIDS RESEARCH, 2007, 35 (11) :3646-3653

[47] A storable encapsulated bilayer chip containing a single protein nanopore [J].

Department of Molecular and Cellular Medicine, Texas A and M University, System Health Science Center, College Station, TX 77843-1114, United States ;

不详 ;

不详 .

J. Am. Chem. Soc., 2007, 15 (4701-4705) :4701-4705

[48] Folding of the thrombin aptamer into a G-quadruplex with Sr2+:: Stability, heat, and hydration [J].

Kankia, BI ;

Marky, LA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (44) :10799-10804

[49] Characterization of individual polynucleotide molecules using a membrane channel [J].

Kasianowicz, JJ ;

Brandin, E ;

Branton, D ;

Deamer, DW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (24) :13770-13773

[50]

Li JWJ, 2002, NANO LETT, V2, P315, DOI [10.1021/nl015713+, 10.1021/n1015713+]

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