Remarkable phosphodiester hydrolysis activity of a novel CeIV complex in neutral aqueous solutions

被引：20

作者：

Baykal, U ^{[1
]}

Akkaya, MS ^{[1
]}

Akkaya, EU ^{[1
]}

机构：

[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

来源：

JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL | 1999年 / 145卷 / 1-2期

关键词：

phosphodiester hydrolysis; artificial enzymes; lanthanide complexes; RNA cleavage;

D O I：

10.1016/S1381-1169(99)00160-0

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel Ce-IV-complex of 1,4,7-tris(carbamoylmethyl)-1,4,7-triazacyclononane has been synthesized. The complex is both stable in aqueous solutions at neutral pH and very efficient in promoting the hydrolysis of a phosphodiester model compound and yeast tRNA(phe). The RNA model compound, 2-hydroxypropyl-p-nitrophenylphosphate hydrolysis is accelerated 7400-fold at pH 7.5, as a result of an unprecedented hydrolytic activity. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：309 / 312

页数：4

共 15 条

[11] IDENTIFICATION OF THE MAGNESIU, EUROPIUM AND LEAD BINDING-SITES IN ESCHERICHIA-COLI AND LUPINE TRANSFER RNAPHE BY SPECIFIC METAL ION-INDUCED CLEAVAGES [J].

MARCINIEC, T ;

CIESIOLKA, J ;

WRZESINSKI, J ;

KRZYZOSIAK, WJ .

FEBS LETTERS, 1989, 243 (02) :293-298

[12] EFFECT OF EUROPIUM(III) ON THERMAL-DENATURATION AND CLEAVAGE OF TRANSFER RIBONUCLEIC-ACIDS [J].

RORDORF, BF ;

KEARNS, DR .

BIOPOLYMERS, 1976, 15 (08) :1491-1504

[13] TETHERING RIBOZYMES TO A RETROVIRAL PACKAGING SIGNAL FOR DESTRUCTION OF VIRAL-RNA [J].

SULLENGER, BA ;

CECH, TR .

SCIENCE, 1993, 262 (5139) :1566-1569

[14] ENORMOUS ACCELERATION BY CERIUM(IV) FOR THE HYDROLYSIS OF NUCLEOSIDE 3',5'-CYCLIC MONOPHOSPHATES AT PH 7 [J].

SUMAOKA, J ;

MIYAMA, S ;

KOMIYAMA, M .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994, (15) :1755-1756

[15] Lanthanide binuclear macrocyclic complexes as synthetic enzymes for the cleavage of DNA [J].

Zhu, B ;

Zhao, DQ ;

Ni, JZ ;

Ying, D ;

Huang, BQ ;

Wang, ZL .

JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL, 1998, 135 (01) :107-110

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