Effects of Nebivolol on Liver Fibrosis Induced by Bile Duct Ligation in Wistar Rats

被引:2
作者
Teixeira, Catarina [1 ]
Franco, Elisabete [1 ]
Oliveira, Paula Alexandra [1 ,6 ]
Colaco, Bruno [2 ,6 ]
Gama, Adelina [1 ,3 ]
Carrola, Joao [4 ]
Pires, Carlos Andre [5 ]
Colaco, Aura [1 ,3 ]
Pires, Maria Joao [1 ,6 ]
机构
[1] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, P-5000911 Vila Real, Portugal
[2] Univ Tras Os Montes & Alto Douro, Dept Zootech, P-5000911 Vila Real, Portugal
[3] Univ Tras Os Montes & Alto Douro, Anim & Vet Res Ctr, P-5000911 Vila Real, Portugal
[4] Univ Tras Os Montes & Alto Douro, Ctr Res & Technol Agroenvironm & Biol Sci, Dept Biol & Environm, P-5000911 Vila Real, Portugal
[5] Univ Tras Os Montes & Alto Douro, Ctr Math, Dept Math, P-5000911 Vila Real, Portugal
[6] Univ Porto, Ctr Study Anim Sci, P-4100 Oporto, Portugal
来源
IN VIVO | 2013年 / 27卷 / 05期
关键词
Liver fibrosis; bile duct ligation; nebivolol; nitric oxide; rat model; HEPATIC STELLATE CELLS; NITRIC-OXIDE; SMOOTH-MUSCLE; ENDOTHELIAL-CELLS; PROLIFERATION; HYPERTENSION; RELAXATIONS; BIOMARKERS; APOPTOSIS;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
100103 [病原生物学]; 100218 [急诊医学];
摘要
Aim: To study the effect of nebivolol on liver fibrosis induced by common bile duct ligation (BDL) in rats. Materials and Methods: After BDL, Wistar rats were divided into three groups (n=24): SO, sham-operated animals; BDL, BDL rats without treatment; BDL+N, BDL rats treated with nebivolol for five weeks. Alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, total bilirubin and albumin levels were assessed. Liver samples collected were stained with hematoxylin-eosin, Masson's trichrom and reticulin. Results: Mortality reached 37.5% in the BDL group, whereas no deaths were observed in the SO and BDL+N groups. The BDL group showed hepatic damage as evidenced by elevation in serum biochemical parameters and fibrosis scores. These pathophysiological changes were attenuated in the BDL+N group. However, there was no significant difference between these two groups. Conclusion: Nebivolol improved the survival rate of animals with BDL, but was unable to significantly improve liver function or reduce liver fibrosis.
引用
收藏
页码:635 / 640
页数:6
相关论文
共 32 条
[1]
Animal models of portal hypertension [J].
Abraldes, Juan G. ;
Pasarin, Marcos ;
Garcia-Pagan, Juan Carlos .
WORLD JOURNAL OF GASTROENTEROLOGY, 2006, 12 (41) :6577-6584
[2]
Nitric oxide augments mesenchymal stem cell ability to repair liver fibrosis [J].
Ali, Gibran ;
Mohsin, Sadia ;
Khan, Mohsin ;
Nasir, Ghazanfar Ali ;
Shams, Sulaiman ;
Khan, Shaheen N. ;
Riazuddin, Sheikh .
JOURNAL OF TRANSLATIONAL MEDICINE, 2012, 10
[3]
Nebivolol induces NO-mediated relaxations of rat small mesenteric but not of large elastic arteries [J].
Altwegg, LA ;
d'Uscio, LV ;
Barandier, C ;
Cosentino, F ;
Yang, ZH ;
Lüscher, TF .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2000, 36 (03) :316-320
[4]
Serum transaminase elevations as indicators of hepatic injury following the administration of drugs [J].
Amacher, DE .
REGULATORY TOXICOLOGY AND PHARMACOLOGY, 1998, 27 (02) :119-130
[5]
Nebivolol inhibits human aortic smooth muscle cell growth:: Effects on cell cycle regulatory proteins [J].
André, DE ;
Arnet, U ;
Yang, ZH ;
Lüscher, TF .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 2000, 35 (06) :845-848
[6]
High doses of olive leaf extract induce liver changes in mice [J].
Arantes-Rodrigues, R. ;
Henriques, A. ;
Pires, M. J. ;
Colaco, B. ;
Calado, A. M. ;
Rema, P. ;
Colaco, A. ;
Fernandes, T. ;
De la Cruz, P. L. F. ;
Lopes, C. ;
Fidalgo-Goncalves, L. ;
Vilela, S. ;
Pedrosa, T. ;
Peixoto, F. ;
Oliveira, P. A. .
FOOD AND CHEMICAL TOXICOLOGY, 2011, 49 (09) :1989-1997
[7]
Progression of liver fibrosis in post-transplant hepatitis C: Mechanisms, assessment and treatment [J].
Berenguer, Marina ;
Schuppan, Detlef .
JOURNAL OF HEPATOLOGY, 2013, 58 (05) :1028-1041
[8]
The management of portal hypertension: Rational basis, available treatments and future options [J].
Bosch, Jaime ;
Berzigotti, Annalisa ;
Garcia-Pagan, Juan Carlos ;
Abraldes, Juan G. .
JOURNAL OF HEPATOLOGY, 2008, 48 :S68-S92
[9]
Effects of nebivolol on proliferation and apoptosis of human coronary artery smooth muscle and endothelial cells [J].
Brehm, BR ;
Wolf, SC ;
Bertsch, D ;
Klaussner, M ;
Wesselborg, S ;
Schüler, S ;
Schulze-Osthoff, K .
CARDIOVASCULAR RESEARCH, 2001, 49 (02) :430-439
[10]
Brehm BR, 2000, J CARDIOVASC PHARM, V36, pS401, DOI 10.1097/00005344-200036001-00117