Immunocytochemical demonstration of androgen receptors in Leydig cells of the bank vole (Clethrionomys glareolus, Schreber): An in vitro study

Androgen receptors of the bank vole Leydig cells in vitro were immunostained using a polyclonal antibody against androgen receptors followed by streptavidine-peroxidase complex or rhodamine-labelled goat anti-rabbit IgG visualization. The immunocytochemical studies revealed localization of androgen receptors in the whole cytoplasm or in the perinuclear area of the cells. Addition of dehydroepiandrosterone into the culture medium resulted in nuclear localization of the androgen receptors. Long (18L:6D) and short (6L: 18D) photoperiods as well as the age of animals were taken into account. The concentration of androgen receptors was changed dependent on age and status of reproduction.