Gas-foamed poly(lactide-co-glycolide) and poly(lactide-co-glycolide) with bioactive glass fibres demonstrate insufficient bone repair in lapine osteochondral defects

被引:13
作者
Salonius, Eve [1 ]
Muhonen, Virpi [1 ]
Lehto, Kalle [2 ]
Jarvinen, Elina [1 ]
Pyhalto, Tuomo [3 ]
Hannula, Markus [2 ]
Aula, Antti S. [2 ,4 ]
Uppstu, Peter [5 ]
Haaparanta, Anne-Marie [2 ]
Rosling, Ari [5 ]
Kellomaki, Minna [2 ]
Kiviranta, Ilkka [1 ,3 ]
机构
[1] Univ Helsinki, Dept Orthopaed & Traumatol, Clinicum, Fac Med, Helsinki, Finland
[2] Tampere Univ Technol, Inst Biosci & Med Technol, BioMediTech, Dept Elect & Commun Engn, Tampere, Finland
[3] Helsinki Univ Hosp, Dept Orthopaed & Traumatol, Helsinki, Finland
[4] Tampere Univ Hosp, Dept Med Phys, Imaging Ctr, Tampere, Finland
[5] Abo Akad Univ, Ctr Excellence Funct Mat Biol Interfaces, Lab Polymer Technol, Turku, Finland
关键词
animal model; biomaterial; bone repair; intra-articular; poly(lactide-co-glycolide); BETA-TRICALCIUM PHOSPHATE; ARTICULAR-CARTILAGE; POROUS SCAFFOLDS; AUTOGENOUS BONE; TISSUE; RABBIT; MODEL; REGENERATION; SUBSTITUTES; STABILITY;
D O I
10.1002/term.2801
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
Deep osteochondral defects may leave voids in the subchondral bone, increasing the risk of joint structure collapse. To ensure a stable foundation for the cartilage repair, bone grafts can be used for filling these defects. Poly(lactide-co-glycolide) (PLGA) is a biodegradable material that improves bone healing and supports bone matrix deposition. We compared the reparative capacity of two investigative macroporous PLGA-based biomaterials with two commercially available bone graft substitutes in the bony part of an intra-articular bone defect created in the lapine femur. New Zealand white rabbits (n = 40) were randomized into five groups. The defects, 4 mm in diameter and 8 mm deep, were filled with neat PLGA; a composite material combining PLGA and bioactive glass fibres (PLGA-BGf); commercial beta-tricalcium phosphate (beta-TCP) granules; or commercial bioactive glass (BG) granules. The fifth group was left untreated for spontaneous repair. After three months, the repair tissue was evaluated with X-ray microtomography and histology. Relative values comparing the operated knee with its contralateral control were calculated. The relative bone volume fraction ( increment BV/TV) was largest in the beta-TCP group (p <= 0.012), which also showed the most abundant osteoid. BG resulted in improved bone formation, whereas defects in the PLGA-BGf group were filled with fibrous tissue. Repair with PLGA did not differ from spontaneous repair. The PLGA, PLGA-BGf, and spontaneous groups showed thicker and sparser trabeculae than the commercial controls. We conclude that bone repair with beta-TCP and BG granules was satisfactory, whereas the investigational PLGA-based materials were only as good as or worse than spontaneous repair.
引用
收藏
页码:406 / 415
页数:10
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