Bioconversion with whole cell penicillin acylase in aqueous two-phase systems

被引：16

作者：

Liao, LC

Ho, CS

Wu, WT ^{[1
]}

机构：

[1] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu, Taiwan

[2] Natl Chiao Tung Univ, Inst Biol Sci & Technol, Hsinchu, Taiwan

来源：

PROCESS BIOCHEMISTRY | 1999年 / 34卷 / 05期

关键词：

penicillin acylase; whole cell; aqueous two-phase system; E; coli; 6-APA; repeated batch;

D O I：

10.1016/S0032-9592(98)00099-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The deacylation of Pen G was carried out by using recombinant E. coli in an aqueous two-phase system consisting of polyethylene glycol and potassium phosphate solution, which partitions the cells to the bottom phase and the products to the top phase. Bioconversion and product separation were carried out in the same reactor. Repeated batch conversion was employed ten times and enzymic activity showed only a slight decline. When pure enzyme was used for bioconversion in an aqueous two-phase system, the decline was fast and bioconversion using whole cell penicillin acylase was better than that obtained using the pure acylase. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：417 / 420

页数：4

共 21 条

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