Colorimetric viral detection based on sialic acid stabilized gold nanoparticles

被引：72

作者：

Lee, Changwon ^{[1
]}

Gaston, Marsha A. ^{[2
]}

Weiss, Alison A. ^{[2
]}

Zhang, Peng ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Chem Biochem & Microbiol, Cincinnati, OH 45221 USA

[2] Univ Cincinnati, Dept Mol Genet Biochem & Microbiol, Cincinnati, OH 45221 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2013年 / 42卷

基金：

美国国家科学基金会;

关键词：

Sialic acid; Au nanoparticle; Viral detection; Colorimetric measurement; INFLUENZA-VIRUS HEMAGGLUTININ; OPTICAL-PROPERTIES; RECEPTOR; BINDING; ASSAY;

D O I：

10.1016/j.bios.2012.10.067

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Sialic acid reduced and stabilized gold nanoparticles (d=20.1 +/- 1.8 nm) were synthesized by a simple one-pot, green method without chemically modifying sialic acid for colorimetric detection of influenza virus. The gold nanoparticles showed target-specific aggregation with viral particles via hemagglutininsialic acid binding. A linear correlation was observed between the change in optical density and dilution of chemically inactivated influenza B/Victoria and influenza B/Yamagata. Virus dilution (hemagglutination assay titer, 512) of 0.156 vol% was readily detected. The upper limit of the linearity can be extended with the use of more sialic acid-gold nanoparticles. (C) 2012 Elsevier B.V. All rights reserved:

引用

页码：236 / 241

页数：6

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