Gold Nanoparticle Arrangement on Viral Particles through Carbohydrate Recognition: A Non-Cross-Linking Approach to Optical Virus Detection

被引：73

作者：

Niikura, Kenichi ^{[1
]}

Nagakawa, Keita ^{[2
]}

Ohtake, Noriko ^{[2
]}

Suzuki, Tadaki ^{[3
]}

Matsuo, Yasutaka ^{[1
]}

Sawa, Hirofumi ^{[3
,4
]}

Ijiro, Kuniharu ^{[1
]}

机构：

[1] Hokkaido Univ, Res Inst Elect Sci, Sapporo, Hokkaido 0010021, Japan

[2] Hokkaido Univ, Grad Sch Sci, Sapporo, Hokkaido 0010021, Japan

[3] Hokkaido Univ, Res Ctr Zoonosis Control, Sapporo, Hokkaido 0010020, Japan

[4] Hokkaido Univ, Global COE Program, Sapporo, Hokkaido 0010020, Japan

来源：

BIOCONJUGATE CHEMISTRY | 2009年 / 20卷 / 10期

关键词：

MOSAIC-VIRUS; BIOCHEMICAL REACTIONS; ORGANIZATION; CELLS;

D O I：

10.1021/bc900255x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We propose a new approach to optical virus detection, based on the spatial assembly of gold nanoparticles on the surface of viruses. Since JC virus-like particles (VLPs) comprise a repeating viral capsid protein that binds to sialic acid, the conjugation of sialic acid-linked Au particles with VLPs enables the spatial arrangement of Au particles on the VLP surface. This structure produced a red shift in the absorption spectrum due to plasmon coupling between adjacent Au particles, leading to the, construction of an optical virus detection system. Our system depends not on the simple cross-linking of VLPs and Au particles, but on an ordered Au structure covering the entire surface of the VLPs and can be applied to virus detection systems using the inherent ligand recognition of animal viruses.

引用

页码：1848 / 1852

页数：5

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