An efficient reproductive method for Irs2-/- mice with C57BL/6JJcl genetic background

被引：4

作者：

Hashimoto, Haruo ^{[1
]}

Eto, Tomoo ^{[1
]}

Kamisako, Tsutomu ^{[1
]}

Hoya, Naoko

Hatakeyama, Teruhiko ^{[1
]}

Arai, Toshiro ^{[2
]}

Yokosuka, Makoto ^{[2
]}

Ohnishi, Yasuyuki ^{[1
]}

Ito, Mamoru ^{[1
]}

Hioki, Kyoji ^{[1
]}

Suzuki, Ryo ^{[3
]}

Ohsugi, Mitsuru ^{[3
]}

Saito, Muneo ^{[1
]}

Ueyama, Yoshito ^{[1
,4
]}

Yamauchi, Toshimasa ^{[3
]}

Kubota, Naoto ^{[3
]}

Tobe, Kazuyuki ^{[3
]}

Kadowaki, Takashi ^{[3
]}

Tamaoki, Norikazu ^{[1
]}

Nomura, Tatsuji ^{[1
]}

Kosaka, Kinori ^{[1
,3
]}

机构：

[1] Cent Inst Expt Anim, Kawasaki, Kanagawa 2160001, Japan

[2] Nippon Vet & Anim Sci Univ, Fac Vet Med, Dept Vet Biochem, Tokyo 1808602, Japan

[3] Univ Tokyo, Grad Sch Med, Dept Metab Dis, Bunkyo Ku, Tokyo 1138655, Japan

[4] Tokai Univ, Sch Med, Dept Pathol, Kanagawa 2591193, Japan

来源：

EXPERIMENTAL ANIMALS | 2008年 / 57卷 / 04期

关键词：

diabetes mellitus; Irs2(-/-) mice; reproduction;

D O I：

10.1538/expanim.57.407

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Efficient reproduction using natural mating and reproduction technology [in vitro fertilization (IVF) and embryo transfer (ET)] was investigated in IRS2 deficient mice with C57BL/6JJcl genetic background (Irs2(-/-) mice) as a typical type 2 diabetes model. From the results using various combinations of Irs2(-/-) and Irs2(-/+) mice, the combination of female Irs2(-/+) x male Irs2(-/-) was found to be more efficient than other combinations. In applications of reproduction technology using IVF and ET, the combination of female Irs2(-/+) x male Irs2(-/-) involves the possibility of Irs2(-/-) production by repeats using female Irs2(-/+) mice. However, reproductive continuity using this combination is difficult because of dependence on human technique and the cost of ET Therefore, we concluded that lrs2(-/-) mice should be produced by embryo transfer using Irs2(-/-) mice from a colony consisting of female Irs2(-/+) x male Irs2(-/-).

引用

页码：407 / 411

页数：5

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