The small viral membrane-associated protein P32 is involved in bacteriophage PRD1 DNA entry

被引:16
作者
Grahn, AM
Daugelavicius, R
Bamford, DH
机构
[1] Univ Helsinki, Viikki Bioctr 2, Dept Biosci, FIN-00014 Helsinki, Finland
[2] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland
[3] Vilnius State Univ, Dept Biochem & Biophys, Vilnius, Lithuania
关键词
D O I
10.1128/JVI.76.10.4866-4872.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The lipid-containing bacteriophage PRDI infects a variety of gram-negative cells by injecting its linear double-stranded DNA genome into the host cell cytoplasm, while the protein capsid is left outside. The virus membrane and several structural proteins are involved in phage DNA entry. In this work we identified a new protein of PRDI. Disruption of gene XXXII resulted in a mutant phenotype defective in phage infectivity reproduction. The absence of the protein P32 did not compromise the particle assembly but led to a defect in phage DNA injection. In P32-deficient particles the phage membrane is unable to undergo a structural transformation from a spherical to a tubular form. Since P32(-) particles are able to increase the permeability of the host cell envelope to a degree comparable to that found with wild-type particles, we suggest that the tail-tube formation is needed to eject the DNA from the phage particle rather than to reach the host cell interior.
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页码:4866 / 4872
页数:7
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