GENOME ORGANIZATION OF MEMBRANE-CONTAINING BACTERIOPHAGE-PRD1

被引:72
作者
BAMFORD, JKH
HANNINEN, AL
PAKULA, TM
OJALA, PM
KALKKINEN, N
FRILANDER, M
BAMFORD, DH
机构
[1] UNIV HELSINKI,DEPT GENET,ARKADIANKATU 7,SF-00100 HELSINKI 10,FINLAND
[2] UNIV HELSINKI,INST BIOTECHNOL,SF-00380 HELSINKI,FINLAND
基金
芬兰科学院;
关键词
D O I
10.1016/0042-6822(91)90995-N
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have determined the nucleotide sequence of the late region (11 kbp) of the lipid-containing bacteriophage PRD1. Gene localization was carried out by complementing nonsense phage mutants with genomic clones containing specific reading frames. The localization was confirmed by sequencing the N-termini of isolated gene products as well as sequencing the N-termini of tryptic fragments of the phage membrane-associated proteins. This, with the previously obtained sequence of the early regions, allowed us to organize most of the phage genes in the phage genome. © 1991.
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页码:658 / 676
页数:19
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