Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin

被引：147

作者：

Ticehurst, JR

Aird, DZ

Dam, LM

Borek, AP

Hargrove, JT

Carroll, KC

机构：

[1] Johns Hopkins Univ Hosp, Div Med Microbiol, Baltimore, MD 21287 USA

[2] Johns Hopkins Bayview Med Ctr, Clin Labs, Baltimore, MD USA

[3] John Hopkins Med, Dept Pathol, Baltimore, MD USA

[4] John Hopkins Med, Dept Med, Baltimore, MD USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 03期

关键词：

D O I：

10.1128/JCM.44.3.1145-1149.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated a two-step algorithm for detecting toxigenic Clostridium difficile: an enzyme immunoassay for glutamate dehydrogenase antigen (Ag-EIA) and then, for antigen-positive specimens, a concurrent cell culture cytotoxicity neutralization assay (CCNA). Antigen-negative results were >= 99% predictive of CCNA negativity. Because the Ag-EIA reduced cell culture workload by approximate to 75 to 80% and two-step testing was complete in <= 3 days, we decided that this algorithm would be effective. Over 6 months, our laboratories' expenses were US$143,000 less than if CCNA alone had been performed on all 5,887 specimens.

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页码：1145 / 1149

页数：5

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