Multilocus microsatellite typing (MLMT) reveals genetic homogeneity of Leishmania donovani strains in the Indian subcontinent

被引:67
作者
Alam, Mohammad Zahangir [1 ,2 ]
Kuhls, Katrin [1 ]
Schweynoch, Carola [1 ]
Sundar, Shyam [3 ]
Rijal, Suman [4 ]
Shamsuzzaman, Abul Khair M. [5 ]
Raju, Balaraju Venkata Subba [6 ]
Salotra, Poonam [6 ]
Dujardin, Jean-Claude [7 ]
Schoenian, Gabriele [1 ]
机构
[1] Charite, Inst Mikrobiol & Hyg, D-10117 Berlin, Germany
[2] Bangladesh Agr Univ, Dept Parasitol, Mymensingh 2202, Bangladesh
[3] Banaras Hindu Univ, Kala Azar Med Res Ctr, Varanasi 221005, Uttar Pradesh, India
[4] BP Koirala Inst Hlth Sci, Dharan, Nepal
[5] Mymensingh Med Coll, Dept Microbiol, Mymensingh, Bangladesh
[6] Indian Council Med Res, Inst Pathol, New Delhi 110029, India
[7] Inst Trop Med, Dept Parasitol, Unit Mol Parasitol, B-2000 Antwerp, Belgium
关键词
Visceral leishmaniasis; Microsatellite; Leishmania donovani; Indian subcontinent; Homogeneity; Population genetics; Epidemiology; VISCERAL LEISHMANIASIS; CUTANEOUS LEISHMANIASIS; POPULATION-STRUCTURE; KALA-AZAR; RESISTANCE; ANTIMONY; MARKERS; NEPAL;
D O I
10.1016/j.meegid.2008.09.005
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
In this population genetic study of Leishmania donovani parasites in the Indian Subcontinent, 132 isolates obtained from patients in Bangladesh, India, Nepal and Sri Lanka suffering from Kala-azar (100), post-Kala-azar dermal leishmaniasis (PKDL) (25) and cutaneous leishmaniasis (CL) (2), and from 5 patients whose clinical patterns were not defined, were analysed by using 15 hyper-variable microsatellite loci. multilocus microsatellite typing (MLMT) data were analysed by using a Bayesian model-based Clustering algorithm and constructing phylogenic tree based on genetic distances. In total, 125 strains from Bangladesh, Bihar (India) and Nepal formed a very homogeneous population regardless of geographical origin, clinical manifestation, and whether they presented in vitro or in Vivo susceptibility to antimonial drugs. Identical multilocus microsatellite profiles were found for 108 strains. other strains differed in only one marker. Considerably, different microsatellite profiles were identified for three Indian strains most closely related to L. donovani from Kenya, and for four strains from Indian and Sri Lankan CL cases. The circulation of a single homogeneous Population of L. donovani in Bihar (India), Bangladesh and Nepal is, most probably, related to the epidemic spread of visceral leishmaniasis in this area, (c) 2008 Elsevier B.V. All rights reserved.
引用
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页码:24 / 31
页数:8
相关论文
共 31 条
[1]  
[Anonymous], 2011, PAUP*: phylogenetic analysis using parsimony
[2]  
Ashford RW, 1986, LEISHMANIA TAXONOMY, P257
[3]   Factors associated with visceral leishmaniasis in Nepal: Bed-net use is strongly protective [J].
Bern, C ;
Joshi, AB ;
Jha, SN ;
Das, ML ;
Hightower, A ;
Thakur, GD ;
Bista, MB .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2000, 63 (3-4) :184-188
[4]  
Bern C, 2006, INDIAN J MED RES, V123, P275
[5]   Comparison of molecular markers for strain typing of Leishmania infantum [J].
Botilde, Yanick ;
Laurent, Thierry ;
Tintaya, Wilber Quispe ;
Chicharro, Carmen ;
Canavate, Carmen ;
Cruz, Israel ;
Kuhls, Katrin ;
Schoenian, Gabriele ;
Dujardin, Jean-Claude .
INFECTION GENETICS AND EVOLUTION, 2006, 6 (06) :440-446
[6]   HIGH-RESOLUTION OF HUMAN EVOLUTIONARY TREES WITH POLYMORPHIC MICROSATELLITES [J].
BOWCOCK, AM ;
RUIZLINARES, A ;
TOMFOHRDE, J ;
MINCH, E ;
KIDD, JR ;
CAVALLISFORZA, LL .
NATURE, 1994, 368 (6470) :455-457
[7]   Gene expression analysis of the mechanism of natural Sb(V) resistance in Leishmania donovani isolates from Nepal [J].
Decuypere, S ;
Rijal, S ;
Yardley, V ;
De Doncker, S ;
Laurent, T ;
Khanal, B ;
Chappuis, F ;
Dujardin, JC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (11) :4616-4621
[8]   Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing [J].
El Tai, NO ;
Osman, OF ;
El Fari, M ;
Presber, W ;
Schönian, G .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2000, 94 (05) :575-579
[9]   Towards a standard battery of microsatellite markers for the analysis of the Leishmania donovani complex [J].
Jamjoom, MB ;
Ashford, RW ;
Bates, PA ;
Kemp, SJ ;
Noyes, HA .
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY, 2002, 96 (03) :265-270
[10]   Lankan cutaneous leishmaniasis is caused by Leishmania donovani zymodeme MON-37 [J].
Karunaweera, ND ;
Pratlong, F ;
Siriwardane, HVYD ;
Ihalamulla, RL ;
Dedet, JP .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2003, 97 (04) :380-381