Visualizing and quantifying adhesive signals

被引：27

作者：

Sabouri-Ghomi, Mohsen ^{[2
]}

Wu, Yi ^{[1
]}

Hahn, Klaus ^{[1
]}

Danuser, Gaudenz ^{[2
]}

机构：

[1] Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA

[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

CURRENT OPINION IN CELL BIOLOGY | 2008年 / 20卷 / 05期

关键词：

D O I：

10.1016/j.ceb.2008.05.004

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Understanding the structural adaptation and signaling of adhesion sites in response to mechanical stimuli requires in situ characterization of the dynamic activation of a large number of adhesion components. Here, we review high-resolution live cell imaging approaches to measure forces, assembly, and interaction of adhesion components, and the activation of adhesion-mediated signals. We conclude by outlining computational multiplexing as a framework for the integration of these data into comprehensive models of adhesion signaling pathways.

引用

页码：541 / 550

页数：10

共 75 条

[1] Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts [J].

Beningo, KA ;

Dembo, M ;

Kaverina, I ;

Small, JV ;

Wang, YL .

JOURNAL OF CELL BIOLOGY, 2001, 153 (04) :881-887

[2] Adhesion-dependent cell mechanosensitivity [J].

Bershadsky, AD ;

Balaban, NQ ;

Geiger, B .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2003, 19 :677-695

[3] Probing the integrin-actin linkage using high-resolution protein velocity mapping [J].

Brown, Claire M. ;

Hebert, Benedict ;

Kolin, David L. ;

Zareno, Jessica ;

Whitmore, Leanna ;

Horwitz, Alan Rick ;

Wiseman, Paul W. .

JOURNAL OF CELL SCIENCE, 2006, 119 (24) :5204-5214

[4] Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains [J].

Burd, CG ;

Emr, SD .

MOLECULAR CELL, 1998, 2 (01) :157-162

[5] Spatial and temporal regulation of focal adhesion kinase activity in living cells [J].

Cai, Xinming ;

Lietha, Daniel ;

Ceccarelli, Derek F. ;

Karginov, Andrei V. ;

Rajfur, Zenon ;

Jacobson, Ken ;

Hahn, Klaus M. ;

Eck, Michael J. ;

Schaller, Michael D. .

MOLECULAR AND CELLULAR BIOLOGY, 2008, 28 (01) :201-214

[6] Spatial distribution and functional significance of activated vinculin in living cells [J].

Chen, H ;

Cohen, DM ;

Choudhury, DM ;

Kioka, N ;

Craig, SW .

JOURNAL OF CELL BIOLOGY, 2005, 169 (03) :459-470

[7] A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows [J].

Chew, TL ;

Wolf, WA ;

Gallagher, PJ ;

Matsumura, F ;

Chisholm, RL .

JOURNAL OF CELL BIOLOGY, 2002, 156 (03) :543-553

[8] FRET detection of cellular α4-integrin conformational activation [J].

Chigaev, A ;

Buranda, T ;

Dwyer, DC ;

Prossnitz, ER ;

Sklar, LA .

BIOPHYSICAL JOURNAL, 2003, 85 (06) :3951-3962

[9] Conformational changes in human integring αIIbβ3 after platelet activation, monitored by FRET [J].

Coutinho, Ana ;

Garcia, Carolina ;

Gonzalez-Rodriguez, Jos ;

Lillo, M. Pilar .

BIOPHYSICAL CHEMISTRY, 2007, 130 (1-2) :76-87

[10] Visual snapshots of intracellular kinase activity at the onset of mitosis [J].

Dai, Zhaohua ;

Dulyaninova, Natalya G. ;

Kumar, Sanjai ;

Bresnick, Anne R. ;

Lawrence, David S. .

CHEMISTRY & BIOLOGY, 2007, 14 (11) :1254-1260

← 1 2 3 4 5 6 7 8 →