Visualizing and quantifying adhesive signals

被引：27

作者：

Sabouri-Ghomi, Mohsen ^{[2
]}

Wu, Yi ^{[1
]}

Hahn, Klaus ^{[1
]}

Danuser, Gaudenz ^{[2
]}

机构：

[1] Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA

[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

CURRENT OPINION IN CELL BIOLOGY | 2008年 / 20卷 / 05期

关键词：

D O I：

10.1016/j.ceb.2008.05.004

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Understanding the structural adaptation and signaling of adhesion sites in response to mechanical stimuli requires in situ characterization of the dynamic activation of a large number of adhesion components. Here, we review high-resolution live cell imaging approaches to measure forces, assembly, and interaction of adhesion components, and the activation of adhesion-mediated signals. We conclude by outlining computational multiplexing as a framework for the integration of these data into comprehensive models of adhesion signaling pathways.

引用

页码：541 / 550

页数：10

共 75 条

[51] High resolution traction force microscopy based on experimental and computational advances [J].

Sabass, Benedikt ;

Gardel, Margaret L. ;

Waterman, Clare M. ;

Schwarz, Ulrich S. .

BIOPHYSICAL JOURNAL, 2008, 94 (01) :207-220

[52] Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [J].

Sakaue-Sawano, Asako ;

Kurokawa, Hiroshi ;

Morimura, Toshifumi ;

Hanyu, Aki ;

Hama, Hiroshi ;

Osawa, Hatsuki ;

Kashiwagi, Saori ;

Fukami, Kiyoko ;

Miyata, Takaki ;

Miyoshi, Hiroyuki ;

Imamura, Takeshi ;

Ogawa, Masaharu ;

Masai, Hisao ;

Miyawaki, Atsushi .

CELL, 2008, 132 (03) :487-498

[53] Fluorescent indicators for Akt/protein kinase B and dynamics of Akt activity visualized in living cells [J].

Sasaki, K ;

Sato, M ;

Umezawa, Y .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (33) :30945-30951

[54] Genetically encoded fluorescent indicators to visualize protein phosphorylation by extracellular signal-regulated kinase in single living cells [J].

Sato, Moritoshi ;

Kawai, Yasutoshi ;

Umezawa, Yoshio .

ANALYTICAL CHEMISTRY, 2007, 79 (06) :2570-2575

[55] Integrins and Src: dynamic duo of adhesion signaling [J].

Shattil, SJ .

TRENDS IN CELL BIOLOGY, 2005, 15 (08) :399-403

[56] Conformational regulation of integrin structure and function [J].

Shimaoka, M ;

Takagi, J ;

Springer, TA .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2002, 31 :485-516

[57] Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells [J].

Stauffer, TP ;

Ahn, S ;

Meyer, T .

CURRENT BIOLOGY, 1998, 8 (06) :343-346

[58] Visualization of synaptic Ca2+/calmodulin-dependent protein kinase II activity in living neurons [J].

Takao, K ;

Okamoto, KI ;

Nakagawa, T ;

Neve, RL ;

Nagai, T ;

Miyawaki, A ;

Hashikawa, T ;

Kobayashi, S ;

Hayashi, Y .

JOURNAL OF NEUROSCIENCE, 2005, 25 (12) :3107-3112

[59] Cells lying on a bed of microneedles: An approach to isolate mechanical force [J].

Tan, JL ;

Tien, J ;

Pirone, DM ;

Gray, DS ;

Bhadriraju, K ;

Chen, CS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (04) :1484-1489

[60] Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells [J].

Ting, AY ;

Kain, KH ;

Klemke, RL ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (26) :15003-15008

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