Chitosan-iron oxide nanobiocomposite based immunosensor for ochratoxin-A

被引：110

作者：

Kaushik, Ajeet ^{[1
,2
]}

Solanki, Pratima R. ^{[1
]}

Ansari, Anees A. ^{[1
]}

Ahmad, Sharif ^{[2
]}

Malhotra, Bansi D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Jamia Millia Islamia, Dept Chem, New Delhi 110025, India

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2008年 / 10卷 / 09期

关键词：

electrochemical immunosensor; Fe3O4; nanoparticles; chitosan; bionanocomposite; ochratoxin-A;

D O I：

10.1016/j.elecom.2008.07.007

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

The rabbit immunoglobulin antibodies (IgGs) have been immobilized onto nanobiocomposite film of chitosan (CH)-iron oxide (Fe3O4) nanoparticles prepared onto indium-tin oxide ([TO) electrode for detection of ochratoxin-A (OTA). Excellent film forming ability and availability of -NH2 group in CH and affinity of surface charged Fe3O4 nanoparticles for oxygen support the immobilization of IgGs. Differential pulse voltammettry (DPV) studies indicate that Fe3O4 nanoparticles provide increased electroactive surface area for loading of IgGs and improved electron transport between IgGs and electrode. IgGs/CH-Fe3O4 nanobiocomposite/ITO immunoelectrode exhibits improved characteristics such as low detection limit (0.5 ng dL(-1)), fast response time (18 s) and high sensitivity (36 mu A/ng dL(-1) cm(-2)) with respect to IgGs/CH/ITO immunoelectrocle. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1364 / 1368

页数：5

共 17 条

[1] Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe3O4 nanoparticles [J].

Cao, DF ;

He, PL ;

Hu, NF .

ANALYST, 2003, 128 (10) :1268-1274

[2] Electrode coatings based on chitosan scaffolds [J].

Cruz, J ;

Kawasaki, M ;

Gorski, W .

ANALYTICAL CHEMISTRY, 2000, 72 (04) :680-686

[3] ATR-FTIR study of IgG adsorbed on different silica surfaces [J].

Giacomelli, CE ;

Bremer, MGEG ;

Norde, W .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1999, 220 (01) :13-23

[4] Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor [J].

Khan, Raju ;

Kaushik, Ajeet ;

Solanki, Pratima R. ;

Ansari, Anees A. ;

Pandey, Manoj K. ;

Malhotra, B. D. .

ANALYTICA CHIMICA ACTA, 2008, 616 (02) :207-213

[5] Nanocrystalline bioactive TiO2-chitosan impedimetric immunosensor for ochratoxin-A [J].

Khan, Raju ;

Dhayal, Marshal .

ELECTROCHEMISTRY COMMUNICATIONS, 2008, 10 (03) :492-495

[6]

Kouassi G. K., 2005, J NANOBIOTECHNOL, V3, P1, DOI DOI 10.1186/1477-3155-3-1

[7] Polysaccharide-modified iron oxide nanoparticles as an effective magnetic affinity adsorbent for bovine serum albumin [J].

Liang, Yuan-Yuan ;

Zhang, Li-Ming ;

Li, Wei ;

Chen, Ru-Fu .

COLLOID AND POLYMER SCIENCE, 2007, 285 (11) :1193-1199

[8] A Fe3O4-based chemical sensor for cathodic determination of hydrogen peroxide [J].

Lin, MS ;

Len, HJ .

ELECTROANALYSIS, 2005, 17 (22) :2068-2073

[9] A REVIEW OF RECENT ADVANCES IN UNDERSTANDING OCHRATOXICOSIS [J].

MARQUARDT, RR ;

FROHLICH, AA .

JOURNAL OF ANIMAL SCIENCE, 1992, 70 (12) :3968-3988

[10] Amperometric hydrogen peroxide biosensor based on immobilization of peroxidase in chitosan matrix crosslinked with glutaraldehyde [J].

Miao, YQ ;

Tan, SN .

ANALYST, 2000, 125 (09) :1591-1594

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