Hypericum perforatum -: Chemical profiling and quantitative results of St. John's Wort products by an improved high-performance liquid chromatography method

被引：67

作者：

Ganzera, M

Zhao, J

Khan, IA ^{[1
]}

机构：

[1] Univ Mississippi, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA

[2] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 2002年 / 91卷 / 03期

关键词：

flavonoids; naphthodianthrones; hyperforin; Hypericum perforatum; HPLC; analysis;

D O I：

10.1002/jps.10057

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The analysis of flavonoids, naphthodianthrones, and the phloroglucinol derivative hyperforin in H. perforatum is described in this article. In a 35-min HPLC run nine major compounds could be identified and baseline separated in the methanolic plant extracts. For an optimum separation the mobile phase consisted of 10 mM ammonium acetate buffer (pH 5.0) and an acetonitrile/methanol mixture; a Synergi MAX-RP 80 Angstrom column (C-12 material) was used as stationary phase. Detection was performed at 270 nm, and the identity of the compounds was confirmed in an LC-MS experiment. Commercial St. John's Wort products were analyzed and qualitative and quantitative results are discussed. (C) 2002 Wiley-Liss, Inc.

引用

页码：623 / 630

页数：8

共 17 条

[1] Identification by high-performance liquid chromatography diode array detection mass spectrometry and quantification by high-performance liquid chromatography UV absorbance detection of active constituents of Hypericum perforatum [J].