The covalently bonded cellulose tris(3,5-dimethylphenylcarbamate) on a silica monolithic capillary column for enantioseparation in capillary electrochromatography

被引：43

作者：

Dong, Xiaoli ^{[1
,2
]}

Wu, Ren'an ^{[1
]}

Dong, Jing ^{[1
]}

Wu, Minghuo ^{[1
]}

Zhu, Yan ^{[2
]}

Zou, Hanfa ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatograph R&A Ctr, Dalian 116023, Peoples R China

[2] Zhejiang Univ, Dept Chem, Hangzhou 310028, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 875卷 / 01期

关键词：

Capillary electrochromatography; Cellulose tris(3,5-dimethylphenylcarbamate); Covalently bonded chiral stationary phase; Enantioseparation; Silica monolithic column;

D O I：

10.1016/j.jchromb.2008.05.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A chiral capillary monolithic column for capillary electrochromatography (CEC) was prepared by covalent bonding of cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) on the silica monolithic matrix within the confine of a 50-mu m i.d. bare fused silica capillary. Several pairs of enantiomers including neutral and basic analytes were baseline resolved on the newly prepared chiral capillary monolithic column in CEC with aqueous mobile phases. Fast enantioseparation was achieved due to the favorable dynamic properties of silica monolith. The covalent bonding of CDMPC as the chiral stationary phase for CEC also enabled the use of THF in mobile phase for enantioseparation of prazquantel by overcoming the incompatibility of THF and the physically coated CDMPC on a column. (c) 2008 Published by Elsevier B.V.

引用

页码：317 / 322

页数：6

共 51 条

[1]

High-performance liquid chromatographic enantioseparations on capillary columns containing monolithic silica modified with amylose tris(3,5-dimethylphenylcarbamate) [J].