Epitope mapping of LB509, a monoclonal antibody directed against human α-synuclein

被引：93

作者：

Jakes, R

Crowther, RA

Lee, VMY

Trojanowski, JQ

Iwatsubo, T

Goedert, M

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA

[3] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Neuropathol & Neurosci, Tokyo, Japan

来源：

NEUROSCIENCE LETTERS | 1999年 / 269卷 / 01期

关键词：

alpha-synuclein; Parkinson's disease; dementia with Lewy bodies; multiple system atrophy; transgenic mice; epitope mapping;

D O I：

10.1016/S0304-3940(99)00411-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

alpha-Synuclein is a 140 amino acid protein that forms the major component of the abnormal filaments that make up the Lewy bod ies and Lewy neu rites of Parkinson's disease a nd dementia with Lewy bodies. It is a Iso the major component of the filamentous glial cytoplasmic inclusions of multiple system atrophy. Here we have used recombinant alpha-synucleins and peptide competition to show that the monoclonal anti-alpha-synuclein antibody LB509 recognizes amino acids 115-122 of human alpha-synuclein. The antibody strongly labelled filaments extracted from multiple system atrophy brain, showing the presence of residues 115-122 of alpha-synuclein. LB509 failed to react with mouse, rat and zebra finch a-synuclein, because of amino acid differences with human alpha-synuclein. Since LB509 recognizes human but not rodent alpha-synuclein, it will be a useful reagent for the characterization of mouse lines transgenic for human a-synuclein. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：13 / 16

页数：4

共 15 条

[1] Baba M, 1998, AM J PATHOL, V152, P879
[2] Synthetic filaments assembled from C-terminally truncated α-synuclein
Crowther, RA
Jakes, R
Spillantini, MG
Goedert, M
[J]. FEBS LETTERS, 1998, 436 (03) : 309 - 312
[3] CHARACTERIZATION OF A NOVEL PROTEIN REGULATED DURING THE CRITICAL PERIOD FOR SONG LEARNING IN THE ZEBRA FINCH
GEORGE, JM
JIN, H
WOODS, WS
CLAYTON, DF
[J]. NEURON, 1995, 15 (02) : 361 - 372
[4] The cDNA cloning and ontogeny of mouse α-synuclein
Hong, L
Ko, HW
Gwag, BJ
Joe, E
Lee, S
Kim, YT
Suh, YH
[J]. NEUROREPORT, 1998, 9 (06) : 1239 - 1243
[5] IDENTIFICATION OF 2 DISTINCT SYNUCLEINS FROM HUMAN BRAIN
JAKES, R
SPILLANTINI, MG
GOEDERT, M
[J]. FEBS LETTERS, 1994, 345 (01) : 27 - 32
[6] Ala30Pro mutation in the gene encoding α-synuclein in Parkinson's disease
Krüger, R
Kuhn, W
Müller, T
Woitalla, D
Graeber, M
Kösel, S
Przuntek, H
Epplen, JT
Schöls, L
Riess, O
[J]. NATURE GENETICS, 1998, 18 (02) : 106 - 108
[7] THE RAT-BRAIN SYNUCLEINS - FAMILY OF PROTEINS TRANSIENTLY ASSOCIATED WITH NEURONAL MEMBRANE
MAROTEAUX, L
SCHELLER, RH
[J]. MOLECULAR BRAIN RESEARCH, 1991, 11 (3-4): : 335 - 343
[8] Mutation in the alpha-synuclein gene identified in families with Parkinson's disease
Polymeropoulos, MH
Lavedan, C
Leroy, E
Ide, SE
Dehejia, A
Dutra, A
Pike, B
Root, H
Rubenstein, J
Boyer, R
Stenroos, ES
Chandrasekharappa, S
Athanassiadou, A
Papapetropoulos, T
Johnson, WG
Lazzarini, AM
Duvoisin, RC
DiIorio, G
Golbe, LI
Nussbaum, RL
[J]. SCIENCE, 1997, 276 (5321) : 2045 - 2047
[9] α-synuclein in filamentous inclusions of Lewy bodies from Parkinson's disease and dementia with Lewy bodies
Spillantini, MG
Crowther, RA
Jakes, R
Hasegawa, M
Goedert, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (11) : 6469 - 6473
[10] alpha-synuclein in Lewy bodies
Spillantini, MG
Schmidt, ML
Lee, VMY
Trojanowski, JQ
Jakes, R
Goedert, M
[J]. NATURE, 1997, 388 (6645) : 839 - 840

← 1 2 →