Detection of miR-106a in gastric carcinoma and its clinical significance

被引：144

作者：

Xiao, Bingxiu ^{[1
]}

Guo, Junming ^{[1
]}

Miao, Ying ^{[1
,2
]}

Jiang, Zhen ^{[1
]}

Huan, Rong ^{[1
,2
]}

Zhang, Yuanyuan ^{[1
]}

Li, Dong ^{[1
]}

Zhong, Jiuchang ^{[1
]}

机构：

[1] Ningbo Univ, Sch Med, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Univ, Sch Med, Ningbo Hosp 2, Affiliated Hosp, Ningbo, Zhejiang, Peoples R China

来源：

CLINICA CHIMICA ACTA | 2009年 / 400卷 / 1-2期

关键词：

Gastric cancer; MicroRNA; miR-106a; Gene diagnosis; MICRORNA EXPRESSION; TUMOR-SUPPRESSOR; LUNG-CANCER; RNA; PROGNOSIS; SIGNATURE; DISCOVERY; DIAGNOSIS; PROFILES; ONCOMIRS;

D O I：

10.1016/j.cca.2008.10.021

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: MicroRNAs (miRNAs) play important roles in carcinogenesis. miRNA-106a (miR-106a) has oncogenic activity in humans, and often has altered expression. The clinical significance of miR-106a in the diagnosis of gastric carcinoma is poorly understood. Methods: The level of miR-106a in 55 gastric carcinoma and 17 non-tumor tissues was quantified by real-time reverse transcriptase-polymerase chain reaction, and the relationship between miR-106a level and clinical and pathological factors was explored. Results: The level of miR-106a in cancer tissues was significantly higher than that in non-tumor tissues, with an average 1.625-fold increase. miR-106a level was significantly associated with tumor stage, size and differentiation; lymphatic and distant metastasis; and invasion (P<0.01). The altered expression of miR-106a was confirmed in gastric cancer cell lines. Conclusion: miR-106a may be a potential biomarker in the diagnosis of gastric carcinoma. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：97 / 102

页数：6

共 40 条

[1] E2F target genes: unraveling the biology [J].

Bracken, AP ;

Ciro, M ;

Cocito, A ;

Helin, K .

TRENDS IN BIOCHEMICAL SCIENCES, 2004, 29 (08) :409-417

[2] MicroRNA signatures in human cancers [J].

Calin, George A. ;

Croce, Carlo M. .

NATURE REVIEWS CANCER, 2006, 6 (11) :857-866

[3]

Chan SH, 2008, ANTICANCER RES, V28, P907

[4]

Chen YJ, 1998, INT J ONCOL, V13, P249

[5] Differential patterns of microRNA expression in neuroblastoma are correlated with prognosis, differentiation, and apoptosis [J].

Chen, Yongxin ;

Stallings, Raymond L. .

CANCER RESEARCH, 2007, 67 (03) :976-983

[6] Nasopharyngeal carcinoma: molecular biomarker discovery and progress [J].

Cho, William Chi-shing .

MOLECULAR CANCER, 2007, 6 (1)

[7] The retinoblastoma tumour suppressor in development and cancer [J].

Classon, M ;

Harlow, E .

NATURE REVIEWS CANCER, 2002, 2 (12) :910-917

[8] Pre-B cell proliferation and lymphoblastic leukemia/high-grade lymphoma in Eμ-miR155 transgenic mice [J].

Costinean, S ;

Zanesi, N ;

Pekarsky, Y ;

Tili, E ;

Volinia, S ;

Heerema, N ;

Croce, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (18) :7024-7029

[9] Deregulated expression of miR-106a predicts survival in human colon cancer patients [J].

Diaz, Raquel ;

Silva, Javier ;

Garcia, Jose M. ;

Lorenzo, Yolanda ;

Garcia, Vanesa ;

Pena, Cristina ;

Rodriguez, Rufo ;

Munoz, Concepcion ;

Garcia, Fernando ;

Bonilla, Felix ;

Dominguez, Gernma .

GENES CHROMOSOMES & CANCER, 2008, 47 (09) :794-802

[10] Evaluation and validation of total RNA extraction methods for MicroRNA expression analyses in formalin-fixed, paraffin-embedded tissues [J].

Doleshal, Martina ;

Magotra, Amber A. ;

Choudhury, Bhavna ;

Cannon, Brian D. ;

Labourier, Emmanuel ;

Szafranska, Anna E. .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2008, 10 (03) :203-211

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