Molecular cloning and characterization of a gibberellin-inducible, putative alpha-glucosidase gene from barley

被引：45

作者：

Tibbot, BK ^{[1
]}

Skadsen, RW ^{[1
]}

机构：

[1] USDA,AGR RES SERV,CEREAL CROPS RES UNIT,MADISON,WI 53705

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 30卷 / 02期

关键词：

alpha-amylase; barley; GA; gene-expression; alpha-glucosidase; maltase;

D O I：

10.1007/BF00020110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A putative alpha-glucosidase clone has been isolated from a cDNA library constructed from mRNA of barley aleurones treated with gibberellin A(3) (GA). The clone is 2752 bp in length and has an uninterrupted open reading frame encoding a polypeptide of 877 amino acids. A 680 amino acid region is 43% identical to human lysosomal alpha-glucosidase and other glycosyl hydrolases. In isolated aleurones, the levels of the corresponding mRNA increase strongly after the application of GA, similar to the pattern exhibited by low-pI alpha-amylase mRNA. High Levels are also observed in the aleurone and scutellum after germination, while low levels are found in developing seeds. The genome contains a single form of this ol-glucosidase gene and two additional sequences that may be related genes or pseudogenes.

引用

页码：229 / 241

页数：13

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