Persistent virus integration into the genome of its algal host, Ectocarpus siliculosus (Phaeophyceae)

被引：50

作者：

Delaroque, N ^{[1
]}

Maier, I ^{[1
]}

Knippers, R ^{[1
]}

Müller, DG ^{[1
]}

机构：

[1] Univ Konstanz, Fak Biol, D-78457 Constance, Germany

来源：

JOURNAL OF GENERAL VIROLOGY | 1999年 / 80卷

关键词：

D O I：

10.1099/0022-1317-80-6-1367

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The brown alga Ectocarpus siliculosus frequently carries an endogenous virus, E, siliculosus virus (EsV-1), the genome of which is a circular, doublestranded DNA molecule of about 320 kbp, After infection, which occurs in the unicellular spores or gametes, the virus is present latently in all somatic cells of the host. Virus multiplication is restricted to cells of the reproductive organs. It has been an open question whether the latent viral DNA occurs as a free episome or becomes integrated into the host genome. PCR studies showed that viral DNA co-migrates with high molecular mass DNA in pulsed-field gel electrophoresis, which confirms that latent viral DNA is integrated into the host genome.

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页码：1367 / 1370

页数：4

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