cellular prion protein;
green fluorescent protein;
microtubules;
kinesin family;
dynein;
D O I:
10.1016/j.bbrc.2004.01.126
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
In order to investigate the microtubule-associated intracellular trafficking of the NH2-terminal cellular prion protein (PrPC) fragment [Biochem. Biophys. Res. Commun. 313 (2004) 818], we performed a real-time imaging of fluorescent PrPC (GFP-PrPC) in living cells. Such GFP-PrPC exhibited an anterograde movement towards the direction of plasma membranes at a speed of 140180 nm/s, and a retrograde movement inwardly at a speed of 1.0-1.2 mum,/s. The anterograde and retrograde movements of GFP-PrPC were blocked by a kinesin family inhibitor (AMP-PNP) and a dynein family inhibitor (vanadate), respectively. Furthermore, anti-kinesin antibody (alpha-kinesin) blocked its anterograde motility, whereas anti-dynein antibody (alpha-dynein) blocked its retrograde motility. These data suggested the kinesin family-driven anterograde and the dynein-driven retrograde movements of GFP-PrPC. Mapping of the interacting domains of PrPC identified amino acid residues indispensable for interactions with kinesin family: NH2-terminal mouse (Mo) residues 53-91 and dynein: NH2-terminal Mo residues 23-33, respectively. Our findings argue that the discrete N-terminal amino acid residues are indispensable for the anterograde and retrograde intracellular movements of PrPC. (C) 2004 Elsevier Inc. All rights reserved.
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页码:802 / 807
页数:6
相关论文
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机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Ivanova, L
;
Barmada, S
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Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Barmada, S
;
Kummer, T
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Kummer, T
;
Harris, DA
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Ivanova, L
;
Barmada, S
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Barmada, S
;
Kummer, T
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
Kummer, T
;
Harris, DA
论文数: 0引用数: 0
h-index: 0
机构:
Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA