Preparation and application of anti-idiotypic antibody against anti-gibberellin A4 antibody

被引：6

作者：

Suzuki, Y ^{[1
]}

Shimada, N ^{[1
]}

Niwa, R ^{[1
]}

Yokoi, M ^{[1
]}

Nakajima, M ^{[1
]}

Murofushi, N ^{[1
]}

Yamaguchi, I ^{[1
]}

机构：

[1] Univ Tokyo, Dept Appl Biol Chem, Div Agr & Agr Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1999年 / 63卷 / 04期

关键词：

anti-idiotypic antibody; ELISA; gibberellin; scFv;

D O I：

10.1271/bbb.63.648

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A monoclonal anti-idiotypic antibody was raised against anti-gibberellin A(4) (GA(4)) antibody, which recognizes biologically active gibberellins such as GA(1) and GA(4) specifically, Amino acid sequences of variable regions of both anti-GA(4) and anti-idiotypic antibodies were analyzed. By using the property of the anti-idiotypic antibody to compete with GA(1/4) in binding to the anti-GA(4) antibody, we successfully applied the anti-idiotypic antibody to ELISA as a tracer for measuring GA(1/4). The single-chain Fv (scFv) gene of the anti-idiotypic antibody was constructed, and scFv expressed in E. coli showed binding activity to anti-GA(4) antibody. These results suggest the possible application of anti-idiotypic antibody as a handy and stable source of an enzymatic tracer for ELISA by production of fusion protein of the scFv and an appropriate enzyme.

引用

页码：648 / 654

页数：7

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