Determination of LSD in blood by capillary electrophoresis with laser-induced fluorescence detection

被引:34
作者
Frost, M
Kohler, H
Blaschke, G
机构
[1] UNIV MUNSTER,INST LEGAL MED,D-48149 MUNSTER,GERMANY
[2] UNIV MUNSTER,INST PHARMACEUT CHEM,D-48149 MUNSTER,GERMANY
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 693卷 / 02期
关键词
LSD; D-lysergic acid diethylamide;
D O I
10.1016/S0378-4347(97)00093-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Capillary electrophoresis (CE) with HeCd laser-induced fluorescence (LIF) detection and its application in forensic toxicology is demonstrated by the determination of D-lysergic acid diethylamide (LSD) in blood. Following precipitation of proteins, washing of the evaporated supernatant and extraction, the residue was reconstituted in methanol and injected electrokinetically (10 s, 10 kV). The total analysis time for quantification of LSD was 8 min using a citrate-methanol buffer, pH 4.0. With this buffer system it is possible to separate LSD, nor-LSD, iso-LSD and iso-nor-LSD. Using a specific sample preparation, electrokinetic injection, extended light path (bubble cell) capillaries and especially LIF detection (lambda(ex) 325 nm, lambda(em) 435 nm), a limit of detection of 0.1-0.2 ng LSD per ml blood could be obtained. The limit of quantitation was about 0.4-0.5 ng/ml. The quantitative evaluation for LSD was carried out using methylergometrine as internal standard. The precision expressed as coefficient of variation (C.V.) and accuracy of the method were <20% and 86-110%, respectively. The application of the method to human blood samples from two forensic cases and a comparison with radioimmunoassay demonstrated that the results were consistent.
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页码:313 / 319
页数:7
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