Active Pt nanoparticles stabilized with glucose oxidase

被引：17

作者：

Karam, Pierre ^{[1
]}

Xin, Yan ^{[2
]}

Jaber, Sarah ^{[1
]}

Halaoui, Lara I. ^{[1
]}

机构：

[1] Amer Univ Beirut, Dept Chem, Beirut 110236, Lebanon

[2] Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32310 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2008年 / 112卷 / 36期

关键词：

D O I：

10.1021/jp800779c

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report the growth of Pt nanoparticles (NP) Coupled and stabilized in situ with glucose oxidase (GOx). The enzyme-capped-Pt nanoparticles were formed from K2PtCl6 (aq) reduced with H-2 (g) in the presence of GOx at a molar ratio of 889/1 Pt(IV)/GOx, creating a direct-linkage between NP and protein during particle growth. Transmission electron microscopy imaging revealed the formation of fcc Pt nanocrystals in the presence of the enzyme with an average dimension < d > = 4.05 +/- 0.62 rim, whereas in its absence Pt black precipitated from Solution. This is evidence that the glycoprotein terminated propagation of Pt growth and stabilized the NP surface. Transfer of Pt NP-GOx to an electrode by assembly oil poly(diallyldimethylammonium chloride) allowed amperometric glucose determination with a low limit of detection of ca. 30 mu M.

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页码：13846 / 13850

页数：5

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