A simplified miRNA-based gene silencing method for Drosophila melanogaster

被引：63

作者：

Haley, Benjamin ^{[1
]}

Hendrix, David ^{[1
]}

Trang, Vinh ^{[1
]}

Levine, Michael ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Div Genet Genom & Dev, Ctr Integrat Genom, Berkeley, CA 94720 USA

来源：

DEVELOPMENTAL BIOLOGY | 2008年 / 321卷 / 02期

关键词：

RNAi; miRNA; decapentaplegic; Nelf; Drosophila; siRNA; shRNA; transgenic; method;

D O I：

10.1016/j.ydbio.2008.06.015

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MicroRNA-based RNA interference is commonly used to produce loss-of-function phenotypes in mammalian systems, but is used only sparingly in invertebrates such as Caenorhabditis elegans and Drosophila melanogaster. Here, we evaluate this method in transgenic strains of D. melanogaster and cultured S2 cells. High throughput-ready expression vectors were developed that permit rapid cloning of synthetic hairpin RNAs. As Proof of concept, this method was used for the efficient silencing of dpp gene activity in the adult wing, and the analysis of the general RNA Polymerase II (Pol II) elongation factor, Nelf-E. Published by Elsevier Inc.

引用

收藏

页码：482 / 490

页数：9

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