Expression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture

被引：34

作者：

Nwe, N

He, QG

Damrongwatanapokin, S

Du, QY

Manopo, I

Limlamthong, Y

Fenner, BJ

Spencer, L

Kwang, J

机构：

[1] Natl Univ Singapore, Temasek Life Sci Lab, Anim Hlth Biotechnol Grp, Singapore 117604, Singapore

[2] Natl Inst Anim Hlth, Dept Livestock Dev, Bangkok, Thailand

来源：

BMC MICROBIOLOGY | 2006年 / 6卷 / 1期

关键词：

D O I：

10.1186/1471-2180-6-16

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine. Results: For vaccine production, hemagglutinin ( HA1) from avian influenza H5N1 was expressed from a recombinant baculovirus. Recombinant HA1 was expressed in monolayer or suspension culture insect cells by infection with the recombinant baculovirus. The yield of rHA1 from the suspension culture was 68 mg/ l, compared to 6 mg/ l from the monolayer culture. Immunization of guinea pigs with 50 mu g of rHA1 yielded hemagglutinin inhibition and virus neutralization titers of 1: 160 after two times vaccination with rHA1 protein. Conclusion: Thus, the production of rHA1 using an insect suspension cell system provides a promising basis for economical production of a H5 antigen.

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页数：7

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