Adsorption/desorption behavior of protein on nanosized hydroxyapatite coatings: A quartz crystal microbalance study

被引：32

作者：

Yang, Zhengpeng ^{[1
]}

Zhang, Chunjing ^{[1
]}

机构：

[1] Henan Polytech Univ, Inst Mat Sci & Engn, Jiaozuo 454000, Peoples R China

来源：

APPLIED SURFACE SCIENCE | 2009年 / 255卷 / 08期

关键词：

Bovine serum albumin; Nanosized hydroxyapatite coatings; Adsorption; Desorption; Quartz crystal microbalance; SERUM-ALBUMIN; ADSORPTION; BIOSENSOR; CHROMATOGRAPHY; SPECTROSCOPY; FIBRINOGEN; SURFACE; PURIFICATION; ELLIPSOMETRY; INTERFACES;

D O I：

10.1016/j.apsusc.2008.11.078

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Protein separation relates closely to the interactions between proteins and various kinds of adsorbents. To obtain a direct and comprehensive understanding of the protein interaction at the solid/solution interface, quartz crystal microbalance (QCM) technique was employed to in situ investigate the adsorption process of bovine serum albumin (BSA) on nanosized hydroxyapatite coatings, and factors affecting its adsorption such as pH, solution ionic strength and temperature were discussed in detail. The adsorption kinetic parameter and the desorption of adsorbed BSA caused by phosphate buffer solution (PBS) introduction were investigated and discussed as well, and an adsorption/desorption mechanism has been proposed. The obtained information suggests that QCM is a useful method for monitoring the adsorption/desorption behavior of BSA on nanosized hydroxyapatite coating. (C) 2008 Elsevier B. V. All rights reserved.

引用

页码：4569 / 4574

页数：6

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